MNB1 Gene is Involved in Regulating the Iron-deficiency Stress Response in Arabidopsis Thaliana

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2022 Mar 29

PMID 35346040

Authors

Hui Song

Feng Chen

Xi Wu

Min Hu

Qingliu Geng

Min Ye

Cheng Zhang

Li Jiang

Shuqing Cao

Affiliations

Soon will be listed here.

Abstract

Background: Iron (Fe) is an essential mineral element that involves in many biological processes important for most plants growth and development. Fe-deficiency induces a complex series of responses in plants, involving physiological and developmental changes, to increase Fe uptake from soil. However, the molecular mechanism involved in plant Fe-deficiency is not well understood.

Results: Here, we found that the MNB1 (mannose-binding-lectin 1) gene is involved in the regulation of Fe-deficiency stress response in Arabidopsis thaliana. The expression abundance of MNB1 was inhibited by Fe-deficiency stress. Knockout of MNB1 led to enhanced Fe accumulation and tolerance, whereas the MNB1-overexpressing plants were sensitive to Fe-deficiency stress. Under conditions of normal and Fe-deficiency, lower HO concentrations were detected in mnb1 mutant plants compared to wild type. On the contrary, higher HO concentrations were found in MNB1-overexpressing plants, which was negatively correlated with malondialdehyde (MDA) levels. Furthermore, in mnb1 mutants, the transcription level of the Fe uptake- and translocation-related genes, FIT, IRT1, FRO2, ZIF, FRD3, NAS4, PYE and MYB72, were considerably elevated during Fe-deficiency stress, resulting in enhanced Fe uptake and translocation, thereby increasing Fe accumulation.

Conclusions: Together, our findings show that the MNB1 gene negatively controls the Fe-deficiency response in Arabidopsis via modulating reactive oxygen species (ROS) levels and the ROS-mediated signaling pathway, thereby affecting the expression of Fe uptake- and translocation-related genes.

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