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Commentary: Dithiothreitol (DTT), When Used As Biofilm Detaching Method to Diagnose Implant-Associated Infections, Does Not Affect Microorganisms' Viability, According to the Current Literature

Overview
Journal Front Microbiol
Specialty Microbiology
Date 2022 Mar 29
PMID 35345543
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References
1.
Karbysheva S, Di Luca M, Butini M, Winkler T, Schutz M, Trampuz A . Comparison of sonication with chemical biofilm dislodgement methods using chelating and reducing agents: Implications for the microbiological diagnosis of implant associated infection. PLoS One. 2020; 15(4):e0231389. PMC: 7141651. DOI: 10.1371/journal.pone.0231389. View

2.
Nielsen H, Hvidt S, Sheils C, Janmey P . Elastic contributions dominate the viscoelastic properties of sputum from cystic fibrosis patients. Biophys Chem. 2004; 112(2-3):193-200. DOI: 10.1016/j.bpc.2004.07.019. View

3.
REEP B, Kaplan W . The use of N-acetyl-L-cysteine and dithiothreitol to process sputa for mycological and fluorescent antibody examinations. Health Lab Sci. 1972; 9(2):118-24. View

4.
Oliva A, Miele M, Al Ismail D, Di Timoteo F, De Angelis M, Rosa L . Challenges in the Microbiological Diagnosis of Implant-Associated Infections: A Summary of the Current Knowledge. Front Microbiol. 2021; 12:750460. PMC: 8586543. DOI: 10.3389/fmicb.2021.750460. View

5.
Shah R, DYE W . Use of dithiothreitol to replace n-acetyl-L-cysteine for routine sputum digestion-decontamination for the culture of mycobacteria. Am Rev Respir Dis. 1966; 94(3):454. DOI: 10.1164/arrd.1966.94.3.454. View