The Proteogenome of Symbiotic in Nodules

Overview

Journal Microorganisms

Specialty Microbiology

Date 2022 Mar 26

PMID 35336227

Authors

Petar Pujic

Nicole Alloisio

Guylaine Miotello

Jean Armengaud

Danis Abrouk

Pascale Fournier

Philippe Normand

Affiliations

Soon will be listed here.

Abstract

Omics are the most promising approaches to investigate microbes for which no genetic tools exist such as the nitrogen-fixing symbiotic A proteogenomic analysis of symbiotic was done by comparing those proteins more and less abundant in nodules relative to N-fixing pure cultures with propionate as the carbon source. There were 250 proteins that were significantly overabundant in nodules at a fold change (FC) ≥ 2 threshold, and 1429 with the same characteristics in in vitro nitrogen-fixing pure culture. Nitrogenase, SuF (Fe-Su biogenesis) and hopanoid lipids synthesis determinants were the most overabundant proteins in symbiosis. Nitrogenase was found to constitute 3% of all proteins in nodules. Sod (superoxide dismutase) was overabundant, indicating a continued oxidative stress, while Kats (catalase) were not. Several transporters were overabundant including one for dicarboxylates and one for branched amino acids. The present results confirm the centrality of nitrogenase in the actinorhizal symbiosis.

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Correction: Pujic et al. The Proteogenome of Symbiotic in Nodules. 2022, , 651.

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