Evaluation of Two Novel Hydantoin Derivatives Using Reconstructed Human Skin Model Episkin: Perspectives for Application As Potential Sunscreen Agents

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Mar 26

PMID 35335215

Authors

Karolina Sloczynska

Justyna Popiol

Agnieszka Gunia-Krzyzak

Paulina Koczurkiewicz-Adamczyk

Pawel Zmudzki

Elzbieta Pekala

Affiliations

Soon will be listed here.

Abstract

This study aimed to assess two novel 5-arylideneimidazolidine-2,4-dione (hydantoin) derivatives (JH3 and JH10) demonstrating photoprotective activity using the reconstructed human skin model Episkin. The skin permeability, irritation, and phototoxicity of the compounds was evaluated in vitro. Moreover, the in vitro genotoxicity and human metabolism of both compounds was studied. For skin permeation and irritation experiments, the test compounds were incorporated into a formulation. It was shown that JH3 and JH10 display no skin irritation and no phototoxicity. Both compounds did not markedly enhance the frequency of micronuclei in CHO-K1 cells in the micronucleus assay. Preliminary in vitro studies with liver microsomes demonstrated that hydrolysis appears to constitute their important metabolic pathway. Episkin permeability experiments showed that JH3 permeability was lower than or close to currently used UV filters, whereas JH10 had the potential to permeate the skin. Therefore, a restriction of this compound permeability should be obtained by choosing the right vehicle or by optimizing it, which should be addressed in future studies.

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