Methods for Vascularization and Perfusion of Tissue Organoids

Overview

Journal Mamm Genome

Specialty Genetics

Date 2022 Mar 25

PMID 35333952

Authors

Hannah A Strobel

Sarah M Moss

James B Hoying

Affiliations

Soon will be listed here.

Abstract

Tissue organoids or "mini organs" can be invaluable tools for understanding health and disease biology, modeling tissue dynamics, or screening potential drug candidates. Effective vascularization of these models is critical for truly representing the in vivo tissue environment. Not only is the formation of a vascular network, and ultimately a microcirculation, essential for proper distribution and exchange of oxygen and nutrients throughout larger organoids, but vascular cells dynamically communicate with other cells to modulate overall tissue behavior. Additionally, interstitial fluid flow, mediated by a perfused microvasculature, can have profound influences on tissue biology. Thus, a truly functionally and biologically relevant organoid requires a vasculature. Here, we review existing strategies for fabricating and incorporating vascular elements and perfusion within tissue organoids.

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