Practical Direct Plaque Assay for Coliphages in 100-ml Samples of Drinking Water

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1986 Sep 1

PMID 3532952

Citations 12

Authors

W O Grabow

P Coubrough

Affiliations

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Abstract

A practical single-agar-layer plaque assay for the direct detection of coliphages in 100-ml samples of water was designed and evaluated. With this assay a 100-ml sample of water, an agar medium containing divalent cations, and the host Escherichia coli C (ATCC 13706) were mixed in a single container, and the mixture was plated on 10 14-cm-diameter petri dishes. It was more sensitive, reliable, and accurate than various other methods and proved rapid, simple, and economic.

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