Screening and Identification of Putative Long Non-Coding RNA in Childhood Obesity: Evaluation of Their Transcriptional Levels

Overview

Journal Biomedicines

Specialty Biomedical Engineering

Date 2022 Mar 25

PMID 35327332

Authors

Manuela Cabiati

Martina Fontanini

Manuel Giacomarra

Gianfranco Politano

Emioli Randazzo

Diego Peroni

Giovanni Federico

Silvia Del Ry

Affiliations

Soon will be listed here.

Abstract

Long non-coding RNAs (LncRNAs) and microRNAs are involved in the pathogenesis of obesity, a multifactorial disease that is characterized by inflammation, cardiometabolic complications, and increased cancer risk among other co-morbidities. The up/down regulation of LncRNAs and microRNAs may play an important role in this condition to identify new diagnostic/prognostic markers. The aim of the study was to identify circulating inflammatory LncRNAs in obese adolescents ( = 54) and to evaluate whether their expression behaved differently compared to normal-weight adolescents ( = 26). To have a more complete insight, the expression of some circulating miRNAs that are linked to obesity () were also analyzed. LncRNAs and miRNAs were extracted simultaneously from plasma samples and amplified by Real-Time PCR. Among the 86 LncRNAs that were analyzed with custom pre-designed plates, only four () were amplified in both normal-weight and obese adolescents and only SNHG12 showed significantly lower expression compared to the normal-weight adolescents ( = 0.026). Circulating miRNAs showed a tendency to increase in obese subjects, except for expression. LncRNAs and miRNAs correlated with some clinical and metabolic parameters. Our results suggest the importance of these new biomarkers to better understand the molecular mechanisms of childhood obesity and its metabolic disorder.

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