The Influence of the Ventricular-Lumbar Gradient on Cerebrospinal Fluid Analysis in Serial Samples

Overview

Journal Brain Sci

Publisher MDPI

Date 2022 Mar 25

PMID 35326365

Authors

Franz Felix Konen

Peter Lange

Ulrich Wurster

Konstantin Fritz Jendretzky

Stefan Gingele

Nora Mohn

Kurt-Wolfram Suhs

Martin Stangel

Thomas Skripuletz

Philipp Schwenkenbecher

Affiliations

Soon will be listed here.

Abstract

Background: Cerebrospinal fluid (CSF) samples from patients with non-inflammatory neurological diseases are used for control groups in biomarker studies. Since large amounts of CSF are withdrawn, patients with idiopathic intracranial hypertension (IIH) or normal pressure hydrocephalus (NPH) are especially suitable. The serially taken CSF portions are usually collected in different tubes. We aimed to investigate whether the later random choice of one of these tubes for CSF investigations might harbor the risk of different CSF protein findings due to the so-called ventriculo-lumbar CSF gradient.

Methods: Patients with IIH (9) and NPH (7) were included. CSF was serially taken and collected in six tubes of 5 mL each. Concentrations and CSF-serum quotients of immunoglobulins, albumin and the virus-specific antibody index (AI) were determined in the first, fourth and sixth CSF fraction.

Results: CSF immunoglobulin and albumin concentrations and CSF-serum protein quotients were significantly lower in the fourth and sixth CSF fraction compared with the first CSF fraction. Virus-specific AI did not significantly differ in the different CSF fractions.

Conclusions: CSF protein analytics should be performed in the first CSF fraction in order to avoid different measurement results and achieve comparability within a control group and between different control and patient groups.

Citing Articles

Differential proteomic profile of lumbar and ventricular cerebrospinal fluid.

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PMID: 36670437 PMC: 9863210. DOI: 10.1186/s12987-022-00405-0.

Concordance of Alzheimer's Disease-Related Biomarkers Between Intraventricular and Lumbar Cerebrospinal Fluid in Idiopathic Normal Pressure Hydrocephalus.

Lukkarinen H, Vanninen A, Tesseur I, Pemberton D, Van Der Ark P, Kokkola T J Alzheimers Dis. 2022; 91(1):305-319.

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