Evidence of Influenza A Virus Infection in Cynomolgus Macaques, Thailand

Overview

Journal Vet Sci

Publisher MDPI

Specialty Veterinary Medicine

Date 2022 Mar 24

PMID 35324860

Authors

Weena Paungpin

Metawee Thongdee

Natthaphat Ketchim

Somjit Chaiwattanarungruengpaisan

Aeknarin Saechin

Ladawan Sariya

Supakarn Kaewchot

Pilaipan Puthavathana

Witthawat Wiriyarat

Affiliations

Soon will be listed here.

Abstract

Little is known about the ecology of influenza A virus (IAV) in nonhuman primates (NHPs). We conducted active surveillance of IAV among 672 cynomolgus macaques () living in 27 free-ranging colonies in Thailand between March and November 2019. A hemagglutination inhibition (HI) assay was employed as the screening test against 16 subtypes of avian influenza virus (AIV) and two strains of the H1 subtype of human influenza virus. The serum samples with HI titers ≥20 were further confirmed by microneutralization (MN) assay. Real-time RT-PCR assay was performed to detect the conserved region of the influenza matrix () gene. The seropositive rate for subtypes of IAV, including AIV H1 (1.6%, 11/672), AIV H2 (15.2%, 102/672), AIV H3 (0.3%, 2/672), AIV H9 (3.4%, 23/672), and human H1 (NP-045) (0.9%, 6/672), was demonstrated. We also found antibody against more than one subtype of IAV in 15 out of 128 positive tested sera (11.7%). Moreover, influenza genome could be detected in 1 out of 245 pool swab samples (0.41%). Evidence of IAV infection presented here emphasizes the role of NHPs in the ecology of the virus. Our findings highlight the need to further conduct a continuous active surveillance program in NHP populations.

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