Data-Independent Acquisition Enables Robust Quantification of 400 Proteins in Non-Depleted Canine Plasma
Overview
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Mass spectrometry-based plasma proteomics offers a major advance for biomarker discovery in the veterinary field, which has traditionally been limited to quantification of a small number of proteins using biochemical assays. The development of foundational data and tools related to sequential window acquisition of all theoretical mass spectra (SWATH)-mass spectrometry has allowed for quantitative profiling of a significant number of plasma proteins in humans and several animal species. Enabling SWATH in dogs enhances human biomedical research as a model species, and significantly improves diagnostic and disease monitoring capability. In this study, a comprehensive peptide spectral library specific to canine plasma proteome was developed and evaluated using SWATH for protein quantification in non-depleted dog plasma. Specifically, plasma samples were subjected to various orthogonal fractionation and digestion techniques, and peptide fragmentation data corresponding to over 420 proteins was collected. Subsequently, a SWATH-based assay was introduced that leveraged the developed resource and that enabled reproducible quantification of 400 proteins in non-depleted plasma samples corresponding to various disease conditions. The ability to profile the abundance of such a significant number of plasma proteins using a single method in dogs has the potential to accelerate biomarker discovery studies in this species.
Characterization of the plasma proteome from healthy adult dogs.
Doulidis P, Kuropka B, Ramos C, Rodriguez-Rojas A, Burgener I Front Vet Sci. 2024; 11:1356318.
PMID: 38638644 PMC: 11024428. DOI: 10.3389/fvets.2024.1356318.
Data Independent Acquisition Reveals In-Depth Serum Proteome Changes in Canine Leishmaniosis.
Martinkovic F, Popovic M, Smolec O, Mrljak V, Eckersall P, Horvatic A Metabolites. 2023; 13(3).
PMID: 36984805 PMC: 10059658. DOI: 10.3390/metabo13030365.