Integrative Insight into Relationships Between Florivorous Thrips and (Insecta, Thysanoptera)

Overview

Journal Insects

Specialty Biology

Date 2022 Mar 24

PMID 35323577

Authors

Agnieszka Kaczmarczyk-Ziemba

Halina Kucharczyk

Marek Kucharczyk

Kinga Kucharska

Affiliations

Soon will be listed here.

Abstract

is recognized as a parthenogenetic form of and is also considered to be a pest in clover-seed plantations. On the contrary, some researchers highlight the distinctiveness of and . Taking into account these two points of view, as well as the lack of molecular studies investigating the relationship between the mentioned thrips, we decided to perform analyses of both mitochondrial (COI) and nuclear markers (28S and ITS2) to determine the genetic diversity of and . Additionally, as a part of an integrative approach, we determined and analyzed their microbiota profiles, based on high-throughput 16S rRNA gene sequencing. The results of the molecular analyses revealed high intraspecific diversity of and did not support the distinctiveness of . The identified microbiota profiles were similar in both species and the performed analyses also did not support the distinctiveness of . Significant differences were, in turn, observed between and larvae. Moreover, two known endosymbiotic bacteria were found in the analyzed microbiota profiles (i.e., and ). Nevertheless, these symbionts were not predominantly found in the bacterial communities that are associated with and thus, its impact on the parthenogenetic mode of its reproduction seems less likely.

References

Gawande S, Anandhan S, Ingle A, Roylawar P, Khandagale K, Gawai T . Microbiome profiling of the onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae). PLoS One. 2019; 14(9):e0223281. PMC: 6768462. DOI: 10.1371/journal.pone.0223281. View

Mound L, Matsunaga J . The species of (Thysanoptera, Phlaeothripinae) and related genera recorded from the Hawaiian Islands. Zookeys. 2017; (662):79-92. PMC: 5539360. DOI: 10.3897/zookeys.662.12107. View

Kadirvel P, Srinivasan R, Hsu Y, Su F, De La Pena R . Application of cytochrome oxidase I sequences for phylogenetic analysis and identification of thrips species occurring on vegetable crops. J Econ Entomol. 2013; 106(1):408-18. DOI: 10.1603/EC12119. View

Asokan R, Krishna Kumar N, Kumar V, Ranganath H . Molecular differences in the mitochondrial cytochrome oxidase I (mtCOI) gene and development of a species-specific marker for onion thrips, Thrips tabaci Lindeman, and melon thrips, T. palmi Karny (Thysanoptera: Thripidae), vectors of tospoviruses.... Bull Entomol Res. 2007; 97(5):461-70. DOI: 10.1017/S0007485307005147. View

Marullo R, Mercati F, Vono G . DNA Barcoding: A Reliable Method for the Identification of Thrips Species (Thysanoptera, Thripidae) Collected on Sticky Traps in Onion Fields. Insects. 2020; 11(8). PMC: 7469196. DOI: 10.3390/insects11080489. View

Huelsenbeck J, Ronquist F . MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics. 2001; 17(8):754-5. DOI: 10.1093/bioinformatics/17.8.754. View

Dickey A, Trease A, Jara-Cavieres A, Kumar V, Christenson M, Potluri L . ESTIMATING BACTERIAL DIVERSITY IN (THYSANOPTERA: THRIPIDAE) VIA NEXT GENERATION SEQUENCING. Fla Entomol. 2014; 97(2):362-366. PMC: 4222051. DOI: 10.1653/024.097.0204. View

Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P . The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res. 2012; 41(Database issue):D590-6. PMC: 3531112. DOI: 10.1093/nar/gks1219. View

Yun J, Roh S, Whon T, Jung M, Kim M, Park D . Insect gut bacterial diversity determined by environmental habitat, diet, developmental stage, and phylogeny of host. Appl Environ Microbiol. 2014; 80(17):5254-64. PMC: 4136111. DOI: 10.1128/AEM.01226-14. View

10.

Arakaki N, Miyoshi T, Noda H . Wolbachia-mediated parthenogenesis in the predatory thrips Franklinothrips vespiformis (Thysanoptera: Insecta). Proc Biol Sci. 2001; 268(1471):1011-6. PMC: 1088702. DOI: 10.1098/rspb.2001.1628. View

11.

Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R . DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol. 1994; 3(5):294-9. View

12.

Kumar V, Dickey A, Seal D, Shatters R, Osborne L, McKenzie C . Unexpected High Intragenomic Variation in Two of Three Major Pest Thrips Species Does Not Affect Ribosomal Internal Transcribed Spacer 2 (ITS2) Utility for Thrips Identification. Int J Mol Sci. 2017; 18(10). PMC: 5666782. DOI: 10.3390/ijms18102100. View

13.

Goujon M, McWilliam H, Li W, Valentin F, Squizzato S, Paern J . A new bioinformatics analysis tools framework at EMBL-EBI. Nucleic Acids Res. 2010; 38(Web Server issue):W695-9. PMC: 2896090. DOI: 10.1093/nar/gkq313. View

14.

Altschul S, Gish W, Miller W, Myers E, Lipman D . Basic local alignment search tool. J Mol Biol. 1990; 215(3):403-10. DOI: 10.1016/S0022-2836(05)80360-2. View

15.

Klindworth A, Pruesse E, Schweer T, Peplies J, Quast C, Horn M . Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. Nucleic Acids Res. 2012; 41(1):e1. PMC: 3592464. DOI: 10.1093/nar/gks808. View

16.

Ghosh A, Jangra S, Dietzgen R, Yeh W . Frontiers Approaches to the Diagnosis of Thrips (Thysanoptera): How Effective Are the Molecular and Electronic Detection Platforms?. Insects. 2021; 12(10). PMC: 8540714. DOI: 10.3390/insects12100920. View

17.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

18.

Minaei K, Mound L . New synonymy in the wheat thrips, Haplothrips tritici (Thysanoptera: Phlaeothripidae). Zootaxa. 2014; (3802):596-9. DOI: 10.11646/zootaxa.3802.4.9. View

19.

Kumm S, Moritz G . First detection of Wolbachia in arrhenotokous populations of thrips species (Thysanoptera: Thripidae and Phlaeothripidae) and its role in reproduction. Environ Entomol. 2009; 37(6):1422-8. DOI: 10.1603/0046-225x-37.6.1422. View

20.

Librado P, Rozas J . DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 2009; 25(11):1451-2. DOI: 10.1093/bioinformatics/btp187. View