Analysis of the Humoral Immunal Response Transcriptome of Infected by

Overview

Journal Insects

Specialty Biology

Date 2022 Mar 24

PMID 35323523

Authors

Yanhua Long

Tian Gao

Song Liu

Yong Zhang

Xiayu Li

Linlin Zhou

Qingqing Su

Letian Xu

Yunqiu Yang

Affiliations

Soon will be listed here.

Abstract

is a destructive masticatory pest in China's tea gardens. as microbial insecticides can effectively control larvae; however, the immune response of this insect infected by are largely unknown. Here, after isolating a highly virulent strain of from , the changes in gene expression among different tissues, including hemocytes and fat bodies, of larvae infected by the entomopathogen were investigated using transcriptome sequencing. A total of 5877 co-expressed differentially expressed genes (DEGs) were identified in hemocytes and fat bodies, of which 5826 were up-regulated in hemocytes and 5784 were up-regulated in fat bodies. We identified 249 immunity-related genes, including pattern recognition receptors, immune effectors, signal modulators, and members of immune pathways. A quantitative real-time PCR analysis confirmed that several pattern recognition receptors were upregulated in hemocytes and fat bodies; however, others were downregulated. The investigated immune effectors ( and ) were suppressed. The results showed that there were tissue differences in the expression of immune genes. This study provides a large number of immunity-related gene sequences from after being infected by , furthering the understanding of the molecular mechanisms of defenses against .

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References

Yu X, Kanost M . Immulectin-2, a lipopolysaccharide-specific lectin from an insect, Manduca sexta, is induced in response to gram-negative bacteria. J Biol Chem. 2000; 275(48):37373-81. DOI: 10.1074/jbc.M003021200. View

Tanaka H, Ishibashi J, Fujita K, Nakajima Y, Sagisaka A, Tomimoto K . A genome-wide analysis of genes and gene families involved in innate immunity of Bombyx mori. Insect Biochem Mol Biol. 2008; 38(12):1087-110. DOI: 10.1016/j.ibmb.2008.09.001. View

Liu J, Ling Z, Wang J, Xiang T, Xu L, Gu C . In vitro transcriptomes analysis identifies some special genes involved in pathogenicity difference of the Beauveria bassiana against different insect hosts. Microb Pathog. 2021; 154:104824. DOI: 10.1016/j.micpath.2021.104824. View

Xia X, Yu L, Xue M, Yu X, Vasseur L, Gurr G . Genome-wide characterization and expression profiling of immune genes in the diamondback moth, Plutella xylostella (L.). Sci Rep. 2015; 5:9877. PMC: 4421797. DOI: 10.1038/srep09877. View

Meng Q, Yu H, Zhang H, Zhu W, Wang M, Zhang J . Transcriptomic insight into the immune defenses in the ghost moth, Hepialus xiaojinensis, during an Ophiocordyceps sinensis fungal infection. Insect Biochem Mol Biol. 2015; 64:1-15. DOI: 10.1016/j.ibmb.2015.06.014. View

Zou Z, Evans J, Lu Z, Zhao P, Williams M, Sumathipala N . Comparative genomic analysis of the Tribolium immune system. Genome Biol. 2007; 8(8):R177. PMC: 2375007. DOI: 10.1186/gb-2007-8-8-r177. View

Li B, Dewey C . RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC Bioinformatics. 2011; 12:323. PMC: 3163565. DOI: 10.1186/1471-2105-12-323. View

Bai J, Xu Z, Li L, Ma W, Xu L, Ma L . Temporospatial modulation of Lymantria dispar immune system against an entomopathogenic fungal infection. Pest Manag Sci. 2020; 76(12):3982-3989. DOI: 10.1002/ps.5947. View

Sutanto K, Husain M, Rasool K, Al-Qahtani W, Aldawood A . Pathogenicity of local and exotic entomopathogenic fungi isolates against different life stages of red palm weevil (Rhynchophorus ferrugineus). PLoS One. 2021; 16(7):e0255029. PMC: 8301634. DOI: 10.1371/journal.pone.0255029. View

10.

Yang Y, Lee S, Nai Y, Kim S, Kim J . Up-regulation of carbon metabolism-related glyoxylate cycle and toxin production in Beauveria bassiana JEF-007 during infection of bean bug, Riptortus pedestris (Hemiptera: Alydidae). Fungal Biol. 2016; 120(10):1236-48. DOI: 10.1016/j.funbio.2016.07.008. View

11.

Gottar M, Gobert V, Matskevich A, Reichhart J, Wang C, Butt T . Dual detection of fungal infections in Drosophila via recognition of glucans and sensing of virulence factors. Cell. 2006; 127(7):1425-37. PMC: 1865096. DOI: 10.1016/j.cell.2006.10.046. View

12.

Dang K, Doggett S, Veera Singham G, Lee C . Insecticide resistance and resistance mechanisms in bed bugs, Cimex spp. (Hemiptera: Cimicidae). Parasit Vectors. 2017; 10(1):318. PMC: 5492349. DOI: 10.1186/s13071-017-2232-3. View

13.

Pan Y, Fang G, Wang Z, Cao Y, Liu Y, Li G . Chromosome-level genome reference and genome editing of the tea geometrid. Mol Ecol Resour. 2021; 21(6):2034-2049. DOI: 10.1111/1755-0998.13385. View

14.

Liu N . Insecticide resistance in mosquitoes: impact, mechanisms, and research directions. Annu Rev Entomol. 2015; 60:537-59. DOI: 10.1146/annurev-ento-010814-020828. View

15.

Lemaitre B, Hoffmann J . The host defense of Drosophila melanogaster. Annu Rev Immunol. 2007; 25:697-743. DOI: 10.1146/annurev.immunol.25.022106.141615. View

16.

Chen A, Wang Y, Shao Y, Zhou Q, Chen S, Wu Y . Genes involved in Beauveria bassiana infection to Galleria mellonella. Arch Microbiol. 2017; 200(4):541-552. DOI: 10.1007/s00203-017-1456-0. View

17.

Gunaratna R, Jiang H . A comprehensive analysis of the Manduca sexta immunotranscriptome. Dev Comp Immunol. 2012; 39(4):388-98. PMC: 3595354. DOI: 10.1016/j.dci.2012.10.004. View

18.

Erasmus R, van den Berg J, du Plessis H . Susceptibility of (Lepidoptera: Gelechiidae) Pupae to Soil Applied Entomopathogenic Fungal Biopesticides. Insects. 2021; 12(6). PMC: 8228705. DOI: 10.3390/insects12060515. View

19.

Kheradmand K, Heidari M, Sedaratian-Jahromi A, Talaei-Hassanloui R, Havasi M . Biological responses of (Acari: Tetranychidae) to sub-lethal concentrations of the entomopathogenic fungus . Bull Entomol Res. 2021; 112(1):70-77. DOI: 10.1017/S0007485321000523. View

20.

Wraight S, Ramos M, Avery P, Jaronski S, Vandenberg J . Comparative virulence of Beauveria bassiana isolates against lepidopteran pests of vegetable crops. J Invertebr Pathol. 2010; 103(3):186-99. DOI: 10.1016/j.jip.2010.01.001. View