Portable FRET-Based Biosensor Device for On-Site Lead Detection

Overview

Journal Biosensors (Basel)

Specialty Biotechnology

Date 2022 Mar 24

PMID 35323427

Authors

Wei-Qun Lai

Yu-Fen Chang

Fang-Ning Chou

De-Ming Yang

Affiliations

Soon will be listed here.

Abstract

Most methods for measuring environmental lead (Pb) content are time consuming, expensive, hazardous, and restricted to specific analytical systems. To provide a facile, safe tool to detect Pb, we created pMet-lead, a portable fluorescence resonance energy transfer (FRET)-based Pb-biosensor. The pMet-lead device comprises a 3D-printed frame housing a 405-nm laser diode-an excitation source for fluorescence emission images (YFP and CFP)-accompanied by optical filters, a customized sample holder with a Met-lead 1.44 M1 (the most recent version)-embedded biochip, and an optical lens aligned for smartphone compatibility. Measuring the emission ratios (Y/C) of the FRET components enabled Pb detection with a dynamic range of nearly 2 (1.96), a pMet-lead/Pb dissociation constant (K) 45.62 nM, and a limit of detection 24 nM (0.474 μg/dL, 4.74 ppb). To mitigate earlier problems with a lack of selectivity for Pb vs. zinc, we preincubated samples with tricine, a low-affinity zinc chelator. We validated the pMet-lead measurements of the characterized laboratory samples and unknown samples from six regions in Taiwan by inductively coupled plasma mass spectrometry (ICP-MS). Notably, two unknown samples had Y/C ratios significantly higher than that of the control (3.48 ± 0.08 and 3.74 ± 0.12 vs. 2.79 ± 0.02), along with Pb concentrations (10.6 ppb and 15.24 ppb) above the WHO-permitted level of 10 ppb in tap water, while the remaining four unknowns showed no detectable Pb upon ICP-MS. These results demonstrate that pMet-lead provides a rapid, sensitive means for on-site Pb detection in water from the environment and in living/drinking supply systems to prevent potential Pb poisoning.

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References

Suwa H, Triller A, Drapeau P, Legendre P . High-affinity zinc potentiation of inhibitory postsynaptic glycinergic currents in the zebrafish hindbrain. J Neurophysiol. 2001; 85(2):912-25. DOI: 10.1152/jn.2001.85.2.912. View

Switz N, DAmbrosio M, Fletcher D . Low-cost mobile phone microscopy with a reversed mobile phone camera lens. PLoS One. 2014; 9(5):e95330. PMC: 4031072. DOI: 10.1371/journal.pone.0095330. View

Zhao W, Tian S, Huang L, Liu K, Dong L, Guo J . A smartphone-based biomedical sensory system. Analyst. 2020; 145(8):2873-2891. DOI: 10.1039/c9an02294e. View

Zhu H, Yaglidere O, Su T, Tseng D, Ozcan A . Cost-effective and compact wide-field fluorescent imaging on a cell-phone. Lab Chip. 2010; 11(2):315-22. PMC: 3073081. DOI: 10.1039/c0lc00358a. View

Caldwell K, Cheng P, Jarrett J, Makhmudov A, Vance K, Ward C . Measurement Challenges at Low Blood Lead Levels. Pediatrics. 2017; 140(2). PMC: 5709716. DOI: 10.1542/peds.2017-0272. View

Leal J, Guerreiro B, Amado P, Fernandes A, Barreira L, Paixao J . On the Development of Selective Chelators for Cadmium: Synthesis, Structure and Chelating Properties of 3-((5-(trifluoromethyl)-1,3,4-thiadiazol-2-yl)amino)benzo[]isothiazole 1,1-dioxide, a Novel Thiadiazolyl Saccharinate. Molecules. 2021; 26(6). PMC: 7999908. DOI: 10.3390/molecules26061501. View

Zhang X, Hu Y, Yang X, Tang Y, Han S, Kang A . FÖrster resonance energy transfer (FRET)-based biosensors for biological applications. Biosens Bioelectron. 2019; 138:111314. DOI: 10.1016/j.bios.2019.05.019. View

Borthwick T, BENSON G, Schugar H . Copper chelating agents. A comparison of cupruretic responses to various tetramines and D-penicillamine. J Lab Clin Med. 1980; 95(4):575-80. View

Knowlton S, Joshi A, Syrrist P, Coskun A, Tasoglu S . 3D-printed smartphone-based point of care tool for fluorescence- and magnetophoresis-based cytometry. Lab Chip. 2017; 17(16):2839-2851. DOI: 10.1039/c7lc00706j. View

10.

Paulson J, Brown M . The CDC blood lead reference value for children: time for a change. Environ Health. 2019; 18(1):16. PMC: 6396537. DOI: 10.1186/s12940-019-0457-7. View

11.

Smith Z, Chu K, Espenson A, Rahimzadeh M, Gryshuk A, Molinaro M . Cell-phone-based platform for biomedical device development and education applications. PLoS One. 2011; 6(3):e17150. PMC: 3047559. DOI: 10.1371/journal.pone.0017150. View

12.

Algar W, Hildebrandt N, Vogel S, Medintz I . FRET as a biomolecular research tool - understanding its potential while avoiding pitfalls. Nat Methods. 2019; 16(9):815-829. DOI: 10.1038/s41592-019-0530-8. View

13.

Yang D, Manurung R, Lin Y, Chiu T, Lai W, Chang Y . Monitoring the Heavy Metal Lead Inside Living Drosophila with a FRET-Based Biosensor. Sensors (Basel). 2020; 20(6). PMC: 7146181. DOI: 10.3390/s20061712. View

14.

Chang T, Lai W, Chang Y, Wang C, Yang D . Development and optimization of heavy metal lead biosensors in biomedical and environmental applications. J Chin Med Assoc. 2021; 84(8):745-753. DOI: 10.1097/JCMA.0000000000000574. View

15.

Calabretta M, Alvarez-Diduk R, Michelini E, Roda A, Merkoci A . Nano-lantern on paper for smartphone-based ATP detection. Biosens Bioelectron. 2019; 150:111902. DOI: 10.1016/j.bios.2019.111902. View

16.

Frank J, Poulakos A, Tornero-Velez R, Xue J . Systematic review and meta-analyses of lead (Pb) concentrations in environmental media (soil, dust, water, food, and air) reported in the United States from 1996 to 2016. Sci Total Environ. 2019; 694:133489. PMC: 6918466. DOI: 10.1016/j.scitotenv.2019.07.295. View

17.

Caldwell K, Cheng P, Vance K, Makhmudov A, Jarrett J, Caudill S . LAMP: A CDC Program to Ensure the Quality of Blood-Lead Laboratory Measurements. J Public Health Manag Pract. 2018; :S23-S30. DOI: 10.1097/PHH.0000000000000886. View

18.

Chiu T, Chen P, Chang C, Yang D . Live-cell dynamic sensing of Cd(2+) with a FRET-based indicator. PLoS One. 2013; 8(6):e65853. PMC: 3679114. DOI: 10.1371/journal.pone.0065853. View

19.

Matsuura H, Yamamoto Y, Muraoka M, Akaishi K, Hori Y, Uemura K . Development of surface-engineered yeast cells displaying phytochelatin synthase and their application to cadmium biosensors by the combined use of pyrene-excimer fluorescence. Biotechnol Prog. 2013; 29(5):1197-202. DOI: 10.1002/btpr.1789. View

20.

Reuben A, Caspi A, Belsky D, Broadbent J, Harrington H, Sugden K . Association of Childhood Blood Lead Levels With Cognitive Function and Socioeconomic Status at Age 38 Years and With IQ Change and Socioeconomic Mobility Between Childhood and Adulthood. JAMA. 2017; 317(12):1244-1251. PMC: 5490376. DOI: 10.1001/jama.2017.1712. View