Inorganic Phosphate in Growing Calcium Carbonate Abalone Shell Suggests a Shared Mineral Ancestral Precursor

Overview

Journal Nat Commun

Specialty Biology

Date 2022 Mar 22

PMID 35314701

Authors

Widad Ajili

Camila B Tovani

Justine Fouassier

Marta de Frutos

Guillaume Pierre Laurent

Philippe Bertani

Chakib Djediat

Frederic Marin

Stephanie Auzoux-Bordenave

Thierry Azais

Nadine Nassif

Affiliations

Soon will be listed here.

Abstract

The presence of phosphate from different origins (inorganic, bioorganic) is found more and more in calcium carbonate-based biominerals. Phosphate is often described as being responsible for the stabilization of the transient amorphous calcium carbonate phase. In order to specify the composition of the mineral phase deposited at the onset of carbonated shell formation, the present study investigates, down to the nanoscale, the growing shell from the European abalone Haliotis tuberculata, using a combination of solid state nuclear magnetic resonance, scanning transmission electron microscope and spatially-resolved electron energy loss spectroscopy techniques. We show the co-occurrence of inorganic phosphate with calcium and carbonate throughout the early stages of abalone shell formation. One possible hypothesis is that this first-formed mixed mineral phase represents the vestige of a shared ancestral mineral precursor that appeared early during Evolution. In addition, our findings strengthen the idea that the final crystalline phase (calcium carbonate or phosphate) depends strongly on the nature of the mineral-associated proteins in vivo.

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