LncSNHG1 Promoted CRC Proliferation Through the MiR-181b-5p/SMAD2 Axis

Overview

Journal J Oncol

Specialty Oncology

Date 2022 Mar 21

PMID 35310912

Authors

Qi Huang

Zhi Yang

Jin-Hai Tian

Pei-Dong You

Jia Wang

Rong Ma

Jingjing Yu

Xu Zhang

Jia Cao

Jie Cao

Li-Bin Wang

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the effects of LncRNA SNHG1 on the proliferation, migration, and epithelial-mesenchymal transition (EMT) of colorectal cancer cells (CRCs).

Methods: 4 pairs of CRC tissue samples and their corresponding adjacent samples were analyzed by the human LncRNA microarray chip. The expression of LncSNHG1 in CRC cell lines was verified by qRT-PCR. Colony formation assays and CCK8 assays were applied to study the changes in cell proliferation. The transwell assay and wound healing experiments were used to verify the cell invasion and migration. EMT progression was confirmed finally.

Results: LncSNHG1 was overexpressed both in CRC tissues and cell lines, while the miR-181b-5p expression was decreased in CRC cell lines. After knock-down of LncSNHG1, the proliferation, invasion, and migration of HT29 and SW620 cells were all decreased. Meanwhile, LncSNHG1 enhanced EMT progress through regulation of the miR-181b-5p/SMAD2 axis.

Conclusion: LncSNHG1 promotes colorectal cancer cell proliferation and invasion through the miR-181b-5p/SMAD2 axis.

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