The Nuclear Skeleton and the Spatial Arrangement of Chromosomes in the Interphase Nucleus of Vertebrate Somatic Cells

Overview

Journal Hum Genet

Specialty Genetics

Date 1986 Sep 1

PMID 3530977

Citations 17

Authors

J Hubert

C A Bourgeois

Affiliations

Soon will be listed here.

Abstract

The topologic distribution of interphase chromosomes established by using various cytologic methods and data concerning the DNA-nuclear skeleton interactions in isolated nuclear fractions were reviewed and discussed. Comparison of these different data clearly showed that the position of chromosomes observed in situ is in agreement with the results obtained from isolated nuclear fractions, indicating that all DNA molecules are bound to the peripheral nuclear skeleton. Moreover, the in situ position of the rDNA near the nuclear envelope can be correlated with the existence of a nucleolar skeleton connected to the peripheral nuclear skeleton. Taking into account the discrepant results regarding the actual existence of an internal nuclear skeleton, we attempted to analyze how the various nuclear skeletal structures described in the literature can be involved in both the distribution of chromosomes and in their chromatin organization. As many questions are still unanswered, we considered the modes of investigation that seem to be the most promising.

Citing Articles

Promoter-Adjacent DNA Hypermethylation Can Downmodulate Gene Expression: in the Muscle Lineage.

Ehrlich K, Lacey M, Baribault C, Sen S, Esteve P, Pradhan S Epigenomes. 2022; 6(4).

PMID: 36547252 PMC: 9778270. DOI: 10.3390/epigenomes6040043.

Replication of centromeric heterochromatin in mouse fibroblasts takes place in early, middle, and late S phase.

Weidtkamp-Peters S, Rahn H, Cardoso M, Hemmerich P Histochem Cell Biol. 2005; 125(1-2):91-102.

PMID: 16231189 DOI: 10.1007/s00418-005-0063-3.

Evidence for a relatively random array of human chromosomes on the mitotic ring.

Allison D, Nestor A J Cell Biol. 1999; 145(1):1-14.

PMID: 10189364 PMC: 2148215. DOI: 10.1083/jcb.145.1.1.

Three-dimensional analysis of the arrangement of compact chromatin in the nucleus of G0 rat lymphocytes.

Lopez-Velazquez G, Marquez J, Ubaldo E, Corkidi G, Echeverria O, Vazquez Nin G Histochem Cell Biol. 1996; 105(2):153-61.

PMID: 8852437 DOI: 10.1007/BF01696155.

Somatic pairing of centromeres and short arms of chromosome 15 in the hematopoietic and lymphoid system.

Lewis J, Tanke H, Raap A, Beverstock G, Kluin-Nelemans H Hum Genet. 1993; 92(6):577-82.

PMID: 8262518 DOI: 10.1007/BF00420942.

References

Miller T, Huang C, Pogo A . Rat liver nuclear skeleton and ribonucleoprotein complexes containing HnRNA. J Cell Biol. 1978; 76(3):675-91. PMC: 2110009. DOI: 10.1083/jcb.76.3.675. View

von Koskull H, AULA P . Nonrandom distribution of chromosome breaks in Fanconi's anemia. Cytogenet Cell Genet. 1973; 12(6):423-34. DOI: 10.1159/000130485. View

Avivi L, Feldman M . Arrangement of chromosomes in the interphase nucleus of plants. Hum Genet. 1980; 55(3):281-95. DOI: 10.1007/BF00290206. View

Bourgeois C, CALVERLEY M, Forman L, POLANI P . Bloom's syndrome: a probable new case with cytogenetic findings. J Med Genet. 1975; 12(4):423-5. PMC: 1013327. DOI: 10.1136/jmg.12.4.423. View

Manuelidis L . Different central nervous system cell types display distinct and nonrandom arrangements of satellite DNA sequences. Proc Natl Acad Sci U S A. 1984; 81(10):3123-7. PMC: 345233. DOI: 10.1073/pnas.81.10.3123. View

Adolph K . Organization of chromosomes in HeLa cells: isolation of histone-depleted nuclei and nuclear scaffolds. J Cell Sci. 1980; 42:291-304. DOI: 10.1242/jcs.42.1.291. View

CASPERSSON T, Haglund U, Lindell B, ZECH L . Radiation-induced non-random chromosome breakage. Exp Cell Res. 1972; 75(2):541-3. DOI: 10.1016/0014-4827(72)90469-7. View

Hsu T, Cooper J, Mace Jr M, Brinkley B . Arrangement of centromeres in mouse cells. Chromosoma. 1971; 34(1):73-87. DOI: 10.1007/BF00285517. View

Schardin M, Cremer T, Hager H, Lang M . Specific staining of human chromosomes in Chinese hamster x man hybrid cell lines demonstrates interphase chromosome territories. Hum Genet. 1985; 71(4):281-7. DOI: 10.1007/BF00388452. View

10.

Beams H, Kessel R . Properties of the Ehrlich ascites tumor cell as determined by electron microscopy, ultracentrifugation, and hydrostatic pressure. Cancer Res. 1968; 28(10):1944-51. View

11.

Aurias A, Dutrillaux B, Buriot D, LeJeune J . High frequencies of inversions and translocations of chromosomes 7 and 14 in ataxia telangiectasia. Mutat Res. 1980; 69(2):369-74. DOI: 10.1016/0027-5107(80)90101-3. View

12.

Robinson S, Nelkin B, Vogelstein B . The ovalbumin gene is associated with the nuclear matrix of chicken oviduct cells. Cell. 1982; 28(1):99-106. DOI: 10.1016/0092-8674(82)90379-8. View

13.

LEWIS C, Lebkowski J, Daly A, Laemmli U . Interphase nuclear matrix and metaphase scaffolding structures. J Cell Sci Suppl. 1984; 1:103-22. DOI: 10.1242/jcs.1984.supplement_1.8. View

14.

Comings D . The rationale for an ordered arrangement of chromatin in the interphase nucleus. Am J Hum Genet. 1968; 20(5):440-60. PMC: 1706348. View

15.

Antoine J, Couturier J, Dutrillaux B . [Non random position of metaphasic chromosomes. II. Effect of condensation on the position of X chromosomes]. Ann Genet. 1982; 25(4):223-5. View

16.

Brenner S . The chromatic nuclear membrane. Exp Cell Res. 1953; 5(1):257-60. DOI: 10.1016/0014-4827(53)90113-x. View

17.

Matsumoto L . Enrichment of satellite DNA on the nuclear matrix of bovine cells. Nature. 1981; 294(5840):481-2. DOI: 10.1038/294481a0. View

18.

Widom J, Klug A . Structure of the 300A chromatin filament: X-ray diffraction from oriented samples. Cell. 1985; 43(1):207-13. DOI: 10.1016/0092-8674(85)90025-x. View

19.

Aaronson R . Isolation of nuclear proteins associated with the nuclear pore complex and the nuclear peripheral lamina of rat liver. Methods Cell Biol. 1977; 16:337-42. DOI: 10.1016/s0091-679x(08)60110-7. View

20.

Hens L, Baumann H, Cremer T, Sutter A, Cornelis J, Cremer C . Immunocytochemical localization of chromatin regions UV-microirradiated in S phase or anaphase. Evidence for a territorial organization of chromosomes during cell cycle of cultured Chinese hamster cells. Exp Cell Res. 1983; 149(1):257-69. DOI: 10.1016/0014-4827(83)90397-x. View