Age- and Hypertension-Related Changes in NOS/NO/sGC-Derived Vasoactive Control of Rat Thoracic Aortae

Overview

Journal Oxid Med Cell Longev

Publisher Wiley

Specialties Cell Biology
Endocrinology

Date 2022 Mar 21

PMID 35308173

Authors

Andrea Berenyiova

Peter Balis

Michal Kluknavsky

Iveta Bernatova

Sona Cacanyiova

Angelika Puzserova

Affiliations

Soon will be listed here.

Abstract

This study was aimed at examining the role of the NOS/NO/sGC signaling pathway in the vasoactive control of the thoracic aorta (TA) from the early to late ontogenetic stages (7 weeks, 20 weeks, and 52 weeks old) of normotensive Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHRs). Systolic blood pressure (SBP) and heart rate (HR) were significantly increased in SHRs compared to age-matched WKYs, which was associated with left heart ventricle hypertrophy in all age groups of rats. The plasma urea level was increased in 20-week-old and 52-week-old SHRs compared with WKYs without increasing creatinine and uric acid. The total cholesterol levels were lower in 20-week-old and 52-week-old SHRs than in WKYs, but triglycerides were higher in 7-week-old SHRs. The fructosamine level was increased in 52-week-old SHRs compared with age-matched WKYs and unchanged in other age groups. Superoxide production was increased only in 7-week-old SHRs compared to age-matched WKYs. The endothelium-dependent relaxation (EDR) of the TA deteriorated in both rat strains during aging; however, endothelial dysfunction already occurred in 20-week-old SHRs and was even more enhanced in 52-week-old rats. Our results also demonstrated increased activity of NOS in 52-week-old WKYs. Moreover, 7-week-old and 52-week-old WKY rats displayed an enhanced residual EDR after L-NMMA (NOS inhibitor) incubation compared with 20-week-old rats. Our results showed that in 7-week-old SHRs, the residual EDR after L-NMMA incubation was increased compared to that in other age groups. The activity of NOS in the TA was comparable in 7-week-old and 20-week-old SHRs, but it was reduced in 52-week-old SHRs compared to younger SHRs and 52-week-old WKYs. Thus, it seems that, in contrast to SHRs, the NOS/NO system in WKYs is probably able to respond to age-related pathologies to maintain endothelial functions and thus optimal BP levels even in later periods of life.

Citing Articles

Ca-Dependent Cl Channels in Vascular Tone Regulation during Aging.

Petrova M, Lassanova M, Tisonova J, Liskova S Int J Mol Sci. 2024; 25(10).

PMID: 38791133 PMC: 11121552. DOI: 10.3390/ijms25105093.

Aging in Normotensive and Spontaneously Hypertensive Rats: Focus on Erythrocyte Properties.

Radosinska J, Kollarova M, Jasenovec T, Radosinska D, Vrbjar N, Balis P Biology (Basel). 2023; 12(7).

PMID: 37508459 PMC: 10376635. DOI: 10.3390/biology12071030.

The Phthalic Selenoanhydride Decreases Rat Blood Pressure and Tension of Isolated Mesenteric, Femoral and Renal Arteries.

Balis P, Berenyiova A, Misak A, Grman M, Rostakova Z, Waczulikova I Molecules. 2023; 28(12).

PMID: 37375381 PMC: 10304488. DOI: 10.3390/molecules28124826.

References

Simao S, Gomes P, Pinto V, Silva E, Amaral J, Igreja B . Age-related changes in renal expression of oxidant and antioxidant enzymes and oxidative stress markers in male SHR and WKY rats. Exp Gerontol. 2011; 46(6):468-74. DOI: 10.1016/j.exger.2011.02.003. View

Slezak P, Puzserova A, Balis P, Sestakova N, Majzunova M, Dovinova I . Genotype-related effect of crowding stress on blood pressure and vascular function in young female rats. Biomed Res Int. 2014; 2014:413629. PMC: 3963217. DOI: 10.1155/2014/413629. View

Matz R, de Sotomayor M, Schott C, STOCLET J, Andriantsitohaina R . Vascular bed heterogeneity in age-related endothelial dysfunction with respect to NO and eicosanoids. Br J Pharmacol. 2000; 131(2):303-11. PMC: 1572322. DOI: 10.1038/sj.bjp.0703568. View

Noale M, Limongi F, Maggi S . Epidemiology of Cardiovascular Diseases in the Elderly. Adv Exp Med Biol. 2020; 1216:29-38. DOI: 10.1007/978-3-030-33330-0_4. View

Balis P, Berenyiova A, Radosinska J, Kvandova M, Bernatova I, Puzserova A . High concentration of uric acid failed to affect endothelial function of small mesenteric arteries, femoral arteries and aortas from aged Wistar-Kyoto rats. J Physiol Pharmacol. 2020; 71(3). DOI: 10.26402/jpp.2020.3.11. View

Miller T, Cherney M, Lee A, Francoleon N, Farmer P, King S . The effects of nitroxyl (HNO) on soluble guanylate cyclase activity: interactions at ferrous heme and cysteine thiols. J Biol Chem. 2009; 284(33):21788-21796. PMC: 2755905. DOI: 10.1074/jbc.M109.014282. View

Puzserova A, Kopincova J, Slezak P, Balis P, Bernatova I . Endothelial dysfunction in femoral artery of the hypertensive rats is nitric oxide independent. Physiol Res. 2013; 62(6):615-29. DOI: 10.33549/physiolres.932517. View

Galvez B, de Castro J, Herold D, Dubrovska G, Arribas S, Gonzalez M . Perivascular adipose tissue and mesenteric vascular function in spontaneously hypertensive rats. Arterioscler Thromb Vasc Biol. 2006; 26(6):1297-302. DOI: 10.1161/01.ATV.0000220381.40739.dd. View

Andrews K, Irvine J, Tare M, Apostolopoulos J, Favaloro J, Triggle C . A role for nitroxyl (HNO) as an endothelium-derived relaxing and hyperpolarizing factor in resistance arteries. Br J Pharmacol. 2009; 157(4):540-50. PMC: 2707966. DOI: 10.1111/j.1476-5381.2009.00150.x. View

10.

Durand M, Castania J, Fazan Jr R, Salgado M, Salgado H . Hemodynamic responses to aortic depressor nerve stimulation in conscious L-NAME-induced hypertensive rats. Am J Physiol Regul Integr Comp Physiol. 2010; 300(2):R418-27. DOI: 10.1152/ajpregu.00463.2010. View

11.

Liskova S, Balis P, Micurova A, Kluknavsky M, Okuliarova M, Puzserova A . Effect of iron oxide nanoparticles on vascular function and nitric oxide production in acute stress-exposed rats. Physiol Res. 2020; 69(6):1067-1083. PMC: 8549880. DOI: 10.33549/physiolres.934567. View

12.

Kozlowska A, Wojtacha P, Rowniak M, Kolenkiewicz M, Tsai M . Differences in serum steroid hormones concentrations in Spontaneously Hypertensive Rats (SHR) - an animal model of Attention-Deficit/Hyperactivity Disorder (ADHD). Physiol Res. 2018; 68(1):25-36. DOI: 10.33549/physiolres.933907. View

13.

Puzserova A, Slezak P, Balis P, Bernatova I . Long-term social stress induces nitric oxide-independent endothelial dysfunction in normotensive rats. Stress. 2012; 16(3):331-9. DOI: 10.3109/10253890.2012.725116. View

14.

Cebova M, Kristek F . Age-dependent ultrastructural changes of coronary artery in spontaneously hypertensive rats. Gen Physiol Biophys. 2011; 30(4):364-72. DOI: 10.4149/gpb_2011_04_364. View

15.

Arishe O, McKenzie J, Priviero F, Ebeigbe A, Webb R . L-arginase induces vascular dysfunction in old spontaneously hypertensive rats. J Afr Assoc Physiol Sci. 2020; 7(2):119-127. PMC: 7051016. View

16.

Cacanyiova S, Berenyiova A, Malekova M, Kristek F, Dovinova I, Krenek P . Different vasoactive effects of chronic endothelial and neuronal NO-synthase inhibition in young Wistar rats. J Physiol Biochem. 2014; 70(3):749-60. DOI: 10.1007/s13105-014-0343-2. View

17.

Puzserova A, Ilovska V, Balis P, Slezak P, Bernatova I . Age-related alterations in endothelial function of femoral artery in young SHR and WKY rats. Biomed Res Int. 2014; 2014:658479. PMC: 3977421. DOI: 10.1155/2014/658479. View

18.

Berenyiova A, Drobna M, Cebova M, Kristek F, Cacanyiova S . Changes in the vasoactive effects of nitric oxide, hydrogen sulfide and the structure of the rat thoracic aorta: the role of age and essential hypertension. J Physiol Pharmacol. 2018; 69(4). DOI: 10.26402/jpp.2018.4.05. View

19.

Kristek F, Drobna M, Cacanyiova S . Different structural alterations in individual conduit arteries of SHRs compared to Wistar rats from the prehypertensive period to late adulthood. Physiol Res. 2017; 66(5):769-780. DOI: 10.33549/physiolres.933690. View

20.

Paolocci N, Jackson M, Lopez B, Miranda K, Tocchetti C, Wink D . The pharmacology of nitroxyl (HNO) and its therapeutic potential: not just the Janus face of NO. Pharmacol Ther. 2007; 113(2):442-58. PMC: 3501193. DOI: 10.1016/j.pharmthera.2006.11.002. View