Effects of Cadmium on Oxidative Stress and Cell Apoptosis in Drosophila Melanogaster Larvae

Overview

Journal Sci Rep

Specialty Science

Date 2022 Mar 21

PMID 35307728

Authors

Pingping Yang

Xingran Yang

Liran Sun

Xiaobing Han

Lu Xu

Wei Gu

Min Zhang

Affiliations

Soon will be listed here.

Abstract

With the increase of human activities, cadmium (Cd) pollution has become a global environmental problem affecting biological metabolism in ecosystem. Cd has a very long half-life in humans and is excreted slowly in organs, which poses a serious threat to human health. In order to better understand the toxicity effects of cadmium, third instar larvae of Drosophila melanogaster (Canton-S strain) were exposed to different concentrations (1.125 mg/kg, 2.25 mg/kg, 4.5 mg/kg, and 9 mg/kg) of cadmium. Trypan blue staining showed that intestinal cell damage of Drosophila larvae increased and the comet assay indicated significantly more DNA damage in larvae exposed to high Cd concentrations. The nitroblue tetrazolium (NBT) experiments proved that content of reactive oxygen species (ROS) increased, which indicated Cd exposure could induce oxidative stress. In addition, the expression of mitochondrial adenine nucleotide transferase coding gene (sesB and Ant2) and apoptosis related genes (Debcl, hid, rpr, p53, Sce and Diap1) changed, which may lead to increased apoptosis. These findings confirmed the toxicity effects on oxidative stress and cell apoptosis in Drosophila larvae after early cadmium exposure, providing insights into understanding the effects of heavy metal stress in animal development.

Citing Articles

Neuroprotective Effects of Morin Against Cadmium- and Arsenic-Induced Cell Damage in PC12 Neurons.

Banaeeyeh S, Razavi B, Hosseinzadeh H Biol Trace Elem Res. 2024; .

PMID: 39436547 DOI: 10.1007/s12011-024-04407-x.

Plastic Fly: What Can Tell Us about the Biological Effects and the Carcinogenic Potential of Nanopolystyrene.

Aloisi M, Grifoni D, Zarivi O, Colafarina S, Morciano P, Poma A Int J Mol Sci. 2024; 25(14).

PMID: 39063206 PMC: 11277132. DOI: 10.3390/ijms25147965.

An overview of the ameliorative efficacy of extract against Cd toxicity: implications for human health and remediation strategies.

Hashim M, Arif H, Tabassum B, Rehman S, Bajaj P, Sirohi R Front Public Health. 2024; 12:1327611.

PMID: 38525339 PMC: 10957771. DOI: 10.3389/fpubh.2024.1327611.

Phenotypical and molecular assessments on the pharmacological effects of curcumin in .

Rumata N, Purwaningsih D, Asbah A, Fadhil Asad M, Chadran D, Emran T Narra J. 2024; 3(2):e117.

PMID: 38454972 PMC: 10919740. DOI: 10.52225/narra.v3i2.117.

Exposure to the Natural Compound Climacostol Induces Cell Damage and Oxidative Stress in the Fruit Fly .

Catalani E, Brunetti K, Del Quondam S, Bongiorni S, Picchietti S, Fausto A Toxics. 2024; 12(2).

PMID: 38393197 PMC: 10891975. DOI: 10.3390/toxics12020102.

References

Song Y, Wang Y, Mao W, Sui H, Yong L, Yang D . Dietary cadmium exposure assessment among the Chinese population. PLoS One. 2017; 12(5):e0177978. PMC: 5436861. DOI: 10.1371/journal.pone.0177978. View

Najafi D, Taheri R, Najafi A, Shamsollahi M, Alvarez-Rodriguez M . Effect of astaxanthin nanoparticles in protecting the post-thawing quality of rooster sperm challenged by cadmium administration. Poult Sci. 2020; 99(3):1678-1686. PMC: 7587804. DOI: 10.1016/j.psj.2019.12.006. View

Augustyniak M, Tarnawska M, Dziewiecka M, Kafel A, Rost-Roszkowska M, Babczynska A . DNA damage in Spodoptera exigua after multigenerational cadmium exposure - A trade-off between genome stability and adaptation. Sci Total Environ. 2020; 745:141048. DOI: 10.1016/j.scitotenv.2020.141048. View

Klatt P, Lamas S . Regulation of protein function by S-glutathiolation in response to oxidative and nitrosative stress. Eur J Biochem. 2000; 267(16):4928-44. DOI: 10.1046/j.1432-1327.2000.01601.x. View

Oswald M, Brooks P, Zwart M, Mukherjee A, West R, Giachello C . Reactive oxygen species regulate activity-dependent neuronal plasticity in . Elife. 2018; 7. PMC: 6307858. DOI: 10.7554/eLife.39393. View

Wang J, Zhang P, Liu N, Wang Q, Luo J, Wang L . Cadmium Induces Apoptosis in Freshwater Crab Sinopotamon henanense through Activating Calcium Signal Transduction Pathway. PLoS One. 2015; 10(12):e0144392. PMC: 4694652. DOI: 10.1371/journal.pone.0144392. View

Pappus S, Mishra M . A Drosophila Model to Decipher the Toxicity of Nanoparticles Taken Through Oral Routes. Adv Exp Med Biol. 2018; 1048:311-322. DOI: 10.1007/978-3-319-72041-8_18. View

Guntur A, Venkatanarayan A, Gangula S, Lundell M . Zfh-2 facilitates Notch-induced apoptosis in the CNS and appendages of Drosophila melanogaster. Dev Biol. 2021; 475:65-79. DOI: 10.1016/j.ydbio.2021.02.009. View

Kulshammer E, Mundorf J, Kilinc M, Frommolt P, Wagle P, Uhlirova M . Interplay among Drosophila transcription factors Ets21c, Fos and Ftz-F1 drives JNK-mediated tumor malignancy. Dis Model Mech. 2015; 8(10):1279-93. PMC: 4610234. DOI: 10.1242/dmm.020719. View

10.

Zhang Y, Wolosker M, Zhao Y, Ren H, Lemos B . Exposure to microplastics cause gut damage, locomotor dysfunction, epigenetic silencing, and aggravate cadmium (Cd) toxicity in Drosophila. Sci Total Environ. 2020; 744:140979. PMC: 8491431. DOI: 10.1016/j.scitotenv.2020.140979. View

11.

Yu L, Chen X, Wei Y, Ding Y, Wang Q, Wang S . Effects of long-term cadmium exposure on trehalose metabolism, growth, and development of Aedes albopictus (Diptera: Culicidae). Ecotoxicol Environ Saf. 2020; 204:111034. DOI: 10.1016/j.ecoenv.2020.111034. View

12.

Sabat D, Patnaik A, Ekka B, Dash P, Mishra M . Investigation of titania nanoparticles on behaviour and mechanosensory organ of Drosophila melanogaster. Physiol Behav. 2016; 167:76-85. DOI: 10.1016/j.physbeh.2016.08.032. View

13.

Hutson R, Thompson R, Bantel A, Tessier C . Acamprosate rescues neuronal defects in the Drosophila model of Fragile X Syndrome. Life Sci. 2018; 195:65-70. DOI: 10.1016/j.lfs.2018.01.007. View

14.

Khatun S, Mandi M, Rajak P, Roy S . Interplay of ROS and behavioral pattern in fluoride exposed Drosophila melanogaster. Chemosphere. 2018; 209:220-231. DOI: 10.1016/j.chemosphere.2018.06.074. View

15.

Anet A, Olakkaran S, Purayil A, Puttaswamygowda G . Bisphenol A induced oxidative stress mediated genotoxicity in Drosophila melanogaster. J Hazard Mater. 2018; 370:42-53. DOI: 10.1016/j.jhazmat.2018.07.050. View

16.

Terhzaz S, Cabrero P, Chintapalli V, Davies S, Dow J . Mislocalization of mitochondria and compromised renal function and oxidative stress resistance in Drosophila SesB mutants. Physiol Genomics. 2009; 41(1):33-41. PMC: 2841493. DOI: 10.1152/physiolgenomics.00147.2009. View

17.

Xin F, Jiang L, Liu X, Geng C, Wang W, Zhong L . Bisphenol A induces oxidative stress-associated DNA damage in INS-1 cells. Mutat Res Genet Toxicol Environ Mutagen. 2014; 769:29-33. DOI: 10.1016/j.mrgentox.2014.04.019. View

18.

Valko M, Morris H, Cronin M . Metals, toxicity and oxidative stress. Curr Med Chem. 2005; 12(10):1161-208. DOI: 10.2174/0929867053764635. View

19.

Zhang Y, Roote J, Brogna S, Davis A, Barbash D, Nash D . stress sensitive B encodes an adenine nucleotide translocase in Drosophila melanogaster. Genetics. 1999; 153(2):891-903. PMC: 1460767. DOI: 10.1093/genetics/153.2.891. View

20.

DeVorkin L, Go N, Hou Y, Moradian A, Morin G, Gorski S . The Drosophila effector caspase Dcp-1 regulates mitochondrial dynamics and autophagic flux via SesB. J Cell Biol. 2014; 205(4):477-92. PMC: 4033768. DOI: 10.1083/jcb.201303144. View