In Vitro Anthelmintic Activity of G. Baker (Annonaceae) Fruits Against

Overview

Journal J Parasit Dis

Specialty Parasitology

Date 2022 Mar 18

PMID 35299909

Authors

Rachel A Nwosu

Mohammed M Suleiman

Hussaina J Makun

Matthew P Ameh

Manji A Shetshak

Isaac O Akefe

Affiliations

Soon will be listed here.

Abstract

Helminthosis is one of the greatest causes of parasitic disease and loss in animal productivity. As such, the control of helminth parasites is of critical importance. This study was aimed to investigate the in vitro anthelmintic activity of G. Baker (Annonaceae) fruits against Using in vitro techniques, the anthelmintic activity of extracts and fractions of G. Baker (Annonaceae) was evaluated for ovicidal (Egg hatch inhibition test) and larvicidal (larval mortality test) activity. Besides, the maximum tolerated dose was determined in adult albino rats administered, 300, 400, and 500 mg/kg body weight of the CME fraction, and observed over a period of 48 h for signs of toxicity and mortality. Phytochemical screening uncovered the occurrence of flavonoids, steroids/triterpenes, cardiac glycosides, saponins, tannins, carbohydrates, and alkaloids in the crude methanol extract (CME), the ethyl acetate fraction (EAF), and butanol fraction. The maximum tolerated dose of the CME of did not produce observable signs of toxicity or death in all the rats given up to 500 mg/kg. The CME and EAF of fruits produced a significant ( ) reduction in the hatchability of eggs in a concentration-dependent manner, while the CME at concentrations between 12.5 and 100 mg/ml completely inhibited the hatching of eggs. Similarly, EAF at doses of 25, 50, and 100 mg/ml completely inhibited the hatching of eggs. The CME and EAF of fruits produced significant ( ) larvicidal activity against L of in a concentration-dependent manner while the CME at concentrations between 6.25 and 100 mg/ml caused larval mortality of L larvae completely. This study suggests that methanol extract and fractions of fruits possess beneficial anthelmintic (ovicidal and larvicidal) activity against , and may be a suitable alternative anthelmintic candidate for the control of nematodes.

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