Development of Novel Markers for Yield in Hevea Brasiliensis Muell. Arg. Based on Candidate Genes from Biosynthetic Pathways Associated with Latex Production

Overview

Journal Biochem Genet

Specialty Molecular Biology

Date 2022 Mar 17

PMID 35296963

Authors

K Bini

Thakurdas Saha

Supriya Radhakrishnan

Minimol Ravindran

Thomas Kadampanattu Uthup

Affiliations

Soon will be listed here.

Abstract

Scarcity of functional genetic markers associated with candidate genes (CGs) is a serious constraint for marker-assisted selection in the natural rubber producing tree, Hevea brasiliensis. In order to develop markers associated with rubber yield, five CGs involved in latex biosynthesis were characterized from 16 popular Hevea varieties. Novel SNPs and indels were identified and developed into markers using simple genotyping techniques like allele-specific PCR, CAPS, etc. A progeny population was genotyped using these markers to validate them, to understand their segregation pattern and to map them to a genetic linkage map. Parent-specific maps were constructed using pseudo-test cross strategy with the help of additional markers. The sequence structure information generated will be useful for future studies on gene mapping, functional relevance of coding SNPs and evolution of rubber biosynthesis genes in Hevea. Concurrently, the markers developed may serve as powerful tools for yield-based selection and for genetic diversity and pedigree studies in Hevea. Above all, the marker assays designed for genotyping could be economically carried out in any laboratory having basic molecular biology infrastructure and expertise.

Citing Articles

The rubber tree kinome: Genome-wide characterization and insights into coexpression patterns associated with abiotic stress responses.

Dos Santos L, Aono A, Francisco F, da Silva C, Souza L, de Souza A Front Plant Sci. 2023; 14:1068202.

PMID: 36824205 PMC: 9941580. DOI: 10.3389/fpls.2023.1068202.

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