Zinc Complex of 3,5-di-tert-butyl Salicylate Inhibits Viability, Migration, and Invasion in Triple-negative Breast Cancer Cells

Overview

Journal Sci Rep

Specialty Science

Date 2022 Mar 17

PMID 35296801

Authors

Heng Chen

Dong Wang

Limei Fan

Zixin Liu

Weiran Zhang

Jinhua Xu

Yunyi Liu

Affiliations

Soon will be listed here.

Abstract

The zinc complex of 3,5-di-tert-butyl salicylate (Zn{[CH)C]Sal}) is a zinc ion chelate of salicylate. In this study, we found that this compound inhibits viability, invasion, and migration and induces apoptosis in triple-negative breast cancer 4T1 cells. RNA-seq showed that the expression of 17 genes was upregulated and 26 genes were downregulated significantly by Zn{[CH)C]Sal} treatment. Further GO and KEGG analysis showed that the activity of Zn{[CH)C]Sal} against triple-negative breast cancer cells may be involved in the JAK-STAT3, HIF-1, and TNF signaling pathways. The expression of key genes was verified by RT-PCR. The phosphorylation of STAT3 and its upstream SRC decreased drastically upon Zn{[CH)C]Sal} treatment, as demonstrated by western blot. Our results indicate that Zn{[CH)C]Sal} inhibits the activity of TNBC cells by downregulating the STAT3 signaling through the SRC pathway.

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