A Model in Female Rats With Phenotypic Features Similar to Interstitial Cystitis/Bladder Pain Syndrome

Overview

Journal Front Pain Res (Lausanne)

Date 2022 Mar 17

PMID 35295535

Authors

Timothy J Ness

Cary DeWitte

Jennifer J Deberry

Morgan P Hart

Buffie Clodfelder-Miller

Jianguo G Gu

Jennifer Ling

Alan Randich

Affiliations

Soon will be listed here.

Abstract

This report describes methodological and exploratory investigations of the zymosan-induced neonatal bladder inflammation (NBI) model of interstitial cystitis/bladder pain syndrome (IC/BPS) in female rats. These results validate and extend the currently employed model by evaluating critical timepoints for obtaining treatment effects and identified that a second insult as an adult including repeat intravesical zymosan, intravesical lipopolysaccharide, acute footshock stress, neuropathic nociception (facial) or somatic inflammation (hindpaw) all resulted in magnified visceromotor responses to urinary bladder distension (UBD) in rats which had experienced NBI when compared with their controls. NBI also resulted in increased tone and reactivity of pelvic floor musculature to UBD, as well as increased responsiveness to intravesical potassium chloride solutions, abnormal anxiety measures (elevated plus maze) and an increased number of submucosal petechial hemorrhages following 30 min of hydrodistension of the bladder. These phenotypic findings have correlates to the clinical features of IC/BPS in humans and so support use of this model system to examine mechanisms of and treatments for IC/BPS.

Citing Articles

Latent bladder hypersensitivity induced by neonatal cystitis in rats unmasked by segmental but not hetero-segmental inflammation.

Ness T, DeWitte C Neurosci Lett. 2024; 846:138090.

PMID: 39701177 PMC: 11700770. DOI: 10.1016/j.neulet.2024.138090.

Neonatal Cystitis Makes Adult Female Rat Urinary Bladders More Sensitive to Low Concentration Microbial Antigens.

Archer A, Deberry J, DeWitte C, Ness T Res Rep Urol. 2023; 15:531-539.

PMID: 38106986 PMC: 10723592. DOI: 10.2147/RRU.S444167.

Animal models of interstitial cystitis/bladder pain syndrome.

Tay C, Grundy L Front Physiol. 2023; 14:1232017.

PMID: 37731545 PMC: 10507411. DOI: 10.3389/fphys.2023.1232017.

Broaden Horizons: The Advancement of Interstitial Cystitis/Bladder Pain Syndrome.

Li J, Yi X, Ai J Int J Mol Sci. 2022; 23(23).

PMID: 36498919 PMC: 9736130. DOI: 10.3390/ijms232314594.

Stress-induced bladder hypersensitivity: Effect of corticotropin releasing factor receptors assessed by spinal neurophysiology and neurochemistry.

Robbins M, DeWitte C, Ness T Neuropharmacology. 2022; 224:109369.

PMID: 36493859 PMC: 9790032. DOI: 10.1016/j.neuropharm.2022.109369.

References

Ness T, DeWitte C, Deberry J, Randich A . Neonatal bladder inflammation alters the role of the central amygdala in hypersensitivity produced by Acute Footshock stress in adult female rats. Brain Res. 2018; 1698:99-105. PMC: 7263074. DOI: 10.1016/j.brainres.2018.06.030. View

Deberry J, Robbins M, Ness T . The amygdala central nucleus is required for acute stress-induced bladder hyperalgesia in a rat visceral pain model. Brain Res. 2015; 1606:77-85. PMC: 4388818. DOI: 10.1016/j.brainres.2015.01.008. View

Miranda A, Mickle A, Schmidt J, Zhang Z, Shaker R, Banerjee B . Neonatal cystitis-induced colonic hypersensitivity in adult rats: a model of viscero-visceral convergence. Neurogastroenterol Motil. 2011; 23(7):683-e281. PMC: 3117950. DOI: 10.1111/j.1365-2982.2011.01724.x. View

Krieger J, Stephens A, Landis J, Clemens J, Kreder K, Lai H . Relationship between chronic nonurological associated somatic syndromes and symptom severity in urological chronic pelvic pain syndromes: baseline evaluation of the MAPP study. J Urol. 2014; 193(4):1254-62. PMC: 4497586. DOI: 10.1016/j.juro.2014.10.086. View

Norwood A, Al-Chaer E, Fantegrossi W . Predisposing effects of neonatal visceral pain on abuse-related effects of morphine in adult male Sprague Dawley rats. Psychopharmacology (Berl). 2014; 231(22):4281-9. PMC: 5384261. DOI: 10.1007/s00213-014-3574-6. View

HAND J . Interstitial cystitis; report of 223 cases (204 women and 19 men). J Urol. 1949; 61(2):291-310. DOI: 10.1016/S0022-5347(17)69067-0. View

Sengupta J, Pochiraju S, Kannampalli P, Bruckert M, Addya S, Yadav P . MicroRNA-mediated GABA Aα-1 receptor subunit down-regulation in adult spinal cord following neonatal cystitis-induced chronic visceral pain in rats. Pain. 2013; 154(1):59-70. PMC: 3535325. DOI: 10.1016/j.pain.2012.09.002. View

Koziol J, Clark D, Gittes R, Tan E . The natural history of interstitial cystitis: a survey of 374 patients. J Urol. 1993; 149(3):465-9. DOI: 10.1016/s0022-5347(17)36120-7. View

Clodfelder-Miller B, Kanda H, Gu J, Creighton J, Ness T, Deberry J . Urothelial bladder afferent neurons in the rat are anatomically and neurochemically distinct from non-urothelial afferents. Brain Res. 2018; 1689:45-53. PMC: 7224329. DOI: 10.1016/j.brainres.2017.12.023. View

10.

Randich A, Mebane H, Ness T . Ice water testing reveals hypersensitivity in adult rats that experienced neonatal bladder inflammation: implications for painful bladder syndrome/interstitial cystitis. J Urol. 2009; 182(1):337-42. DOI: 10.1016/j.juro.2009.02.107. View

11.

Peters K, Killinger K, A Ibrahim I . Childhood symptoms and events in women with interstitial cystitis/painful bladder syndrome. Urology. 2008; 73(2):258-62. DOI: 10.1016/j.urology.2008.09.014. View

12.

Coutinho S, Meller S, Gebhart G . Intracolonic zymosan produces visceral hyperalgesia in the rat that is mediated by spinal NMDA and non-NMDA receptors. Brain Res. 1996; 736(1-2):7-15. DOI: 10.1016/0006-8993(96)00661-0. View

13.

Kanda H, Ling J, Chang Y, Erol F, Viatchenko-Karpinski V, Yamada A . Kv4.3 Channel Dysfunction Contributes to Trigeminal Neuropathic Pain Manifested with Orofacial Cold Hypersensitivity in Rats. J Neurosci. 2021; 41(10):2091-2105. PMC: 8018767. DOI: 10.1523/JNEUROSCI.2036-20.2021. View

14.

Lin C, Al-Chaer E . Long-term sensitization of primary afferents in adult rats exposed to neonatal colon pain. Brain Res. 2003; 971(1):73-82. DOI: 10.1016/s0006-8993(03)02358-8. View

15.

Zhang J, Yu J, Kannampalli P, Nie L, Meng H, Medda B . MicroRNA-mediated downregulation of potassium-chloride-cotransporter and vesicular γ-aminobutyric acid transporter expression in spinal cord contributes to neonatal cystitis-induced visceral pain in rats. Pain. 2017; 158(12):2461-2474. PMC: 5680103. DOI: 10.1097/j.pain.0000000000001057. View

16.

Deberry J, Randich A, Shaffer A, Robbins M, Ness T . Neonatal bladder inflammation produces functional changes and alters neuropeptide content in bladders of adult female rats. J Pain. 2009; 11(3):247-55. PMC: 2835826. DOI: 10.1016/j.jpain.2009.07.010. View

17.

Castroman P, Ness T . Vigor of visceromotor responses to urinary bladder distension in rats increases with repeated trials and stimulus intensity. Neurosci Lett. 2001; 306(1-2):97-100. DOI: 10.1016/s0304-3940(01)01886-9. View

18.

Al-Chaer E, Kawasaki M, Pasricha P . A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation during postnatal development. Gastroenterology. 2000; 119(5):1276-85. DOI: 10.1053/gast.2000.19576. View

19.

Sutcliffe S, Colditz G, Goodman M, Pakpahan R, Vetter J, Ness T . Urological chronic pelvic pain syndrome symptom flares: characterisation of the full range of flares at two sites in the Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network. BJU Int. 2014; 114(6):916-25. PMC: 4198521. DOI: 10.1111/bju.12778. View

20.

Randich A, Uzzell T, Cannon R, Ness T . Inflammation and enhanced nociceptive responses to bladder distension produced by intravesical zymosan in the rat. BMC Urol. 2006; 6:2. PMC: 1395324. DOI: 10.1186/1471-2490-6-2. View