Molecular Characterization of Novel Genotypes in from Uruguay

Overview

Journal Curr Res Parasitol Vector Borne Dis

Date 2022 Mar 14

PMID 35284857

Authors

Maria L Felix

Sebastian Munoz-Leal

Luis A Carvalho

Diego Queirolo

Susana Remesar Alonso

Santiago Nava

Maria T Armua-Fernandez

Jose M Venzal

Affiliations

Soon will be listed here.

Abstract

are small intracellular Gram-negative bacteria transmitted by ticks. These microorganisms cause ehrlichiosis, a complex of life-threatening emerging zoonoses and diseases of global veterinary relevance. The aim of this study was to investigate the presence of in free-living collected in Uruguay. Ticks were collected from vegetation in five localities from the southeast and northeast of the country between 2014 and 2017. Detection of DNA was performed in pools of adults or nymphs grouped according to the collection site and date. A total of 1,548 ticks were collected in four of the five locations sampled. Fragments of three loci ( rRNA, and ) were obtained by PCR, and phylogenies inferred using Bayesian inference analysis for each gene independently. DNA of spp. was found in 15 out of 42 tick pools. Based on the topology of the phylogenetic trees, our sequences represent two novel genotypes for the genus named as sp. Serrana and sp. Laguna Negra. Both genotypes were closely related to sp. Magellanica, a species detected in and Magellanic penguins. Considering that all stages of and are parasites of birds, their phylogenetic relationships, and common eco-epidemiological profiles, it is reasonable to state that these genotypes of spp. may represent a natural group likely associated with birds. Our results constitute the first characterization of spp. in Uruguay. Future studies on birds reported as hosts for are needed to further understand the epidemiological cycles of both genotypes in the country. Finally, does not feed on humans, so the two species reported herein might have no implications in human health.

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