WNK3-PER1 Interactions Regulate the Circadian Rhythm in the Suprachiasmatic Nucleus in Rats

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2022 Mar 11

PMID 35273702

Authors

Zhao-Huan Zhang

Jian-Mei Xiong

Yun-Yi Zhu

Xiao-Dan Zhang

Wen-Jie Wu

Lin Zhou

Jian-Hua Zhuang

Xiao-Hui Xu

Affiliations

Soon will be listed here.

Abstract

PER1 is a core component of the internal time-keeping system. In the suprachiasmatic nucleus, it serves as the primary circadian pacemaker in mammalian brains. PER1 functions with other clock components to generate a feedback loop involving the transcriptional repression of gene expression to produce a circadian rhythm with an approximately 24-hour cycle. Post-transcriptional modifications (PTMs) are a basic regulatory mechanism that both perpetuate self-sustained oscillations and interpret metabolic input into circadian physiology by affecting factors such as protein stability, interactions, localization, and activity. Here we examined whether the serine/threonine protein kinase WNK3, which is expressed in a circadian rhythm, can interact and colocalize with PER1 in the SCN. In rats, WNK3 knockdown in the SCN is associated with altered sleep patterns. Moreover, WNK3 can phosphorylate PER1 to promote its degradation and is associated with circadian oscillations when PER1 is expressed in vitro.

Citing Articles

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