BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 Module Positively Contributes to Resistance in

Overview

Journal Plants (Basel)

Date 2022 Mar 10

PMID 35270079

Authors

Ka Zhang

Chenjian Zhuo

Zhixin Wang

Fei Liu

Jing Wen

Bin Yi

Jinxiong Shen

Chaozhi Ma

Tingdong Fu

Jinxing Tu

Affiliations

Soon will be listed here.

Abstract

(oilseed rape) is one of the most important oil crops worldwide, but its growth is seriously threatened by . The mechanism of oilseed rape response to this pathogen has rarely been studied. Here, it was identified that BnaA03.MKK5 whose expression was induced by infection was involved in plant immunity. overexpression lines exhibited decreased disease symptoms compared to wild-type plants, accompanied by the increased expression of camalexin-biosynthesis-related genes, including and . In addition, two copies of ( and ) were induced by incubation, and BnaA03.MKK5 interacted with BnaA06.MPK3/BnaC03.MPK3 in yeast. These interactions were confirmed using in vivo co-immunoprecipitation assays. In vitro phosphorylation assays showed that BnaA06.MPK3 and BnaC03.MPK3 were the direct phosphorylation substrates of BnaA03.MKK5. The transgenic oilseed rape plants including and overexpression lines and gene editing lines mediated by CRISPR/Cas9 were generated; the results of the genetic transformation of indicate that BnMPK3 also has a positive role in resistance. This study provides information about the potential mechanism of defense against mediated by a detailed BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 module.

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