Characterization of the Wheat Heat Shock Factor TaHsfA2e-5D Conferring Heat and Drought Tolerance in

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Mar 10

PMID 35269925

Authors

Huihui Bi

Jingnan Miao

Jinqiu He

Qifan Chen

Jiajun Qian

Huanhuan Li

Yan Xu

Dan Ma

Yue Zhao

Xuejun Tian

Wenxuan Liu

Affiliations

Soon will be listed here.

Abstract

Environmental stresses, especially heat and drought, severely limit plant growth and negatively affect wheat yield and quality worldwide. Heat shock factors (Hsfs) play a central role in regulating plant responses to various stresses. In this study, the wheat heat shock factor gene on chromosome 5D was isolated and functionally characterized, with the goal of investigating its role in responses to heat and drought stresses. Gene expression profiling showed that was expressed constitutively in various wheat tissues, most highly in roots at the reproductive stage. The expression of was highly up-regulated in wheat seedlings by heat, cold, drought, high salinity, and multiple phytohormones. The TaHsfA2e-5D protein was localized in the nucleus and showed a transcriptional activation activity. Ectopic expression of the in yeast exhibited improved thermotolerance. Overexpression of the in results in enhanced tolerance to heat and drought stresses. Furthermore, RT-qPCR analyses revealed that functions through increasing the expression of Hsp genes and other stress-related genes, including and . Collectively, these results suggest that functions as a positive regulator of plants' responses to heat and drought stresses, which may be of great significance for understanding and improving environmental stress tolerance in crops.

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