Encodes an Essential Mitochondrial Splicing Cofactor Required for MRNA Processing and Embryo Development in

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Mar 10

PMID 35269810

Authors

Tan-Trung Nguyen

Corinne Best

Sofia Shevtsov

Michal Zmudjak

Martine Quadrado

Ron Mizrahi

Hagit Zer

Hakim Mireau

Oren Ostersetzer-Biran

Affiliations

Soon will be listed here.

Abstract

Mitochondria play key roles in cellular energy metabolism in eukaryotes. Mitochondria of most organisms contain their own genome and specific transcription and translation machineries. The expression of angiosperm mtDNA involves extensive RNA-processing steps, such as RNA trimming, editing, and the splicing of numerous group II-type introns. Pentatricopeptide repeat (PPR) proteins are key players in plant organelle gene expression and RNA metabolism. In the present analysis, we reveal the function of the 2 gene (, ) and show that it encodes a mitochondria-localized PPR protein that is crucial for early embryo development in . Molecular characterization of embryo-rescued plantlets indicates that the splicing of intron 1, and thus respiratory complex I biogenesis, are strongly compromised. Moreover, the molecular function seems conserved between MISF2 protein in and its orthologous gene () in maize, suggesting that the ancestor of MISF2/EMP10 was recruited to function in processing before the monocot-dicot divergence ~200 million years ago. These data provide new insights into the function of nuclear-encoded factors in mitochondrial gene expression and respiratory chain biogenesis during plant embryo development.

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