Expression and Transcript Localization of , and in the Early Brain of the Orange-Spotted Grouper

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Mar 10

PMID 35269757

Authors

Ganesan Nagarajan

Adimoolam Aruna

Yousef Ahmed Alkhamis

Roshmon Thomas Mathew

Ching-Fong Chang

Affiliations

Soon will be listed here.

Abstract

We investigated the developmental expression and localization of and transcripts in the brain of the juvenile orange-spotted grouper in response to steroidogenic enzyme gene at various developmental ages in relation to gonadal sex differentiation. The transcripts were significantly higher from 110-dah (day after hatching) and gradually increased up to 150-dah. The mRNA, on the other hand, showed a decreased expression during this period, in contrast to expression. At the same time, the early brain had increased levels of steroidogenic gene (). and hybridization signals were found to be increased in the ventromedial hypothalamus at 110-dah; however, mRNA signals decreased in the early brain toward 150-dah. Furthermore, the exogenous estradiol upregulated and transcripts in the early brain of the grouper. These findings suggest that and may have an antagonistic expression pattern in the early brain during gonadal sex differentiation. Increased expression of steroidogenic gene together with during gonadal differentiation strongly suggests that may play an important role in the juvenile grouper brain steroidogenesis and brain development.

Citing Articles

Localization of the Neuropeptide Arginine Vasotocin and Its Receptor in the Osmoregulatory Organs of Black Porgy, : Gills, Kidneys, and Intestines.

Nagarajan G, Adimoolam A, Alkhamis Y, Mathew R, Chang C Int J Mol Sci. 2022; 23(21).

PMID: 36362208 PMC: 9658894. DOI: 10.3390/ijms232113421.

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