CabHLH79 Acts Upstream of to Regulate Cold Stress in Pepper

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Mar 10

PMID 35269676

Authors

Ziyu Wang

Yumeng Zhang

Huifang Hu

Lang Chen

Huafeng Zhang

Rugang Chen

Affiliations

Soon will be listed here.

Abstract

Cold stress is one of the main restricting factors affecting plant growth and agricultural production. Complex cold signaling pathways induce the expression of hundreds of cold-sensitive genes. The NAC transcription factor has previously been reported to significantly influence the response of pepper to cold stress. Here, using Yeast one-hybrid (Y1H) library screened to search for other relevant molecular factors, we identified that directly binds to the promoter. Different basic helix-loop-helix (bHLH) transcription factors (TFs) in plants significantly respond to multiple plant stresses, but the mechanism of bHLHs in the cold tolerance of pepper is still unclear. This study investigated the functional characterization of in the regulation of cold resistance in pepper. Down-regulation of in pepper by virus-induced gene silencing (VIGS) increased its sensitivity to low temperature, whereas overexpression of in pepper or enhanced cold resistance. Compared with control plants, VIGS mediated of had lower enzyme activity and related gene expression levels, accompanied by higher reactive oxygen species (ROS) accumulation, relative electrolyte leakage (REL), and malondialdehyde accumulation (MDA) contents. Transient overexpression of pepper positively regulated cold stress response genes and ROS genes, which reduced REL and MDA contents. Similarly, ectopic expression of in showed less ROS accumulation, and higher enzymes activities and expression levels. These results indicated that enhanced cold tolerance by enhancing the expression of ROS-related and other cold stress tolerance-related genes. Taken together, our results showed a multifaceted module of bHLH79-NAC035 in the cold stress of pepper.

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References

Clough S, Bent A . Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1999; 16(6):735-43. DOI: 10.1046/j.1365-313x.1998.00343.x. View

Chinnusamy V, Ohta M, Kanrar S, Lee B, Hong X, Agarwal M . ICE1: a regulator of cold-induced transcriptome and freezing tolerance in Arabidopsis. Genes Dev. 2003; 17(8):1043-54. PMC: 196034. DOI: 10.1101/gad.1077503. View

Yu X, Liu Y, Wang S, Tao Y, Wang Z, Shu Y . CarNAC4, a NAC-type chickpea transcription factor conferring enhanced drought and salt stress tolerances in Arabidopsis. Plant Cell Rep. 2015; 35(3):613-27. DOI: 10.1007/s00299-015-1907-5. View

Thomashow M . PLANT COLD ACCLIMATION: Freezing Tolerance Genes and Regulatory Mechanisms. Annu Rev Plant Physiol Plant Mol Biol. 2004; 50:571-599. DOI: 10.1146/annurev.arplant.50.1.571. View

Yamaguchi-Shinozaki K, Shinozaki K . Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annu Rev Plant Biol. 2006; 57:781-803. DOI: 10.1146/annurev.arplant.57.032905.105444. View

Zhang Z, Chen J, Liang C, Liu F, Hou X, Zou X . Genome-Wide Identification and Characterization of the bHLH Transcription Factor Family in Pepper ( L.). Front Genet. 2020; 11:570156. PMC: 7545091. DOI: 10.3389/fgene.2020.570156. View

Atchley W, Terhalle W, Dress A . Positional dependence, cliques, and predictive motifs in the bHLH protein domain. J Mol Evol. 1999; 48(5):501-16. DOI: 10.1007/pl00006494. View

Suzuki N, Koussevitzky S, Mittler R, Miller G . ROS and redox signalling in the response of plants to abiotic stress. Plant Cell Environ. 2011; 35(2):259-70. DOI: 10.1111/j.1365-3040.2011.02336.x. View

Puranik S, Sahu P, Srivastava P, Prasad M . NAC proteins: regulation and role in stress tolerance. Trends Plant Sci. 2012; 17(6):369-81. DOI: 10.1016/j.tplants.2012.02.004. View

10.

Verma D, Jalmi S, Bhagat P, Verma N, Sinha A . A bHLH transcription factor, MYC2, imparts salt intolerance by regulating proline biosynthesis in Arabidopsis. FEBS J. 2019; 287(12):2560-2576. DOI: 10.1111/febs.15157. View

11.

Wei K, Chen H . Comparative functional genomics analysis of bHLH gene family in rice, maize and wheat. BMC Plant Biol. 2018; 18(1):309. PMC: 6267037. DOI: 10.1186/s12870-018-1529-5. View

12.

Li C, Yan C, Sun Q, Wang J, Yuan C, Mou Y . The bHLH transcription factor AhbHLH112 improves the drought tolerance of peanut. BMC Plant Biol. 2021; 21(1):540. PMC: 8594184. DOI: 10.1186/s12870-021-03318-6. View

13.

Cai W, Yang S, Wu R, Cao J, Shen L, Guan D . Pepper NAC-type transcription factor NAC2c balances the trade-off between growth and defense responses. Plant Physiol. 2021; 186(4):2169-2189. PMC: 8331138. DOI: 10.1093/plphys/kiab190. View

14.

Chinnusamy V, Zhu J, Zhu J . Cold stress regulation of gene expression in plants. Trends Plant Sci. 2007; 12(10):444-51. DOI: 10.1016/j.tplants.2007.07.002. View

15.

Ke Y, Wu Y, Zhou H, Chen P, Wang M, Liu M . Genome-wide survey of the bHLH super gene family in Brassica napus. BMC Plant Biol. 2020; 20(1):115. PMC: 7071649. DOI: 10.1186/s12870-020-2315-8. View

16.

Li F, Guo S, Zhao Y, Chen D, Chong K, Xu Y . Overexpression of a homopeptide repeat-containing bHLH protein gene (OrbHLH001) from Dongxiang Wild Rice confers freezing and salt tolerance in transgenic Arabidopsis. Plant Cell Rep. 2010; 29(9):977-86. DOI: 10.1007/s00299-010-0883-z. View

17.

Arkus K, Cahoon E, Jez J . Mechanistic analysis of wheat chlorophyllase. Arch Biochem Biophys. 2005; 438(2):146-55. DOI: 10.1016/j.abb.2005.04.019. View

18.

Liu H, Yang Y, Liu D, Wang X, Zhang L . Transcription factor TabHLH49 positively regulates dehydrin WZY2 gene expression and enhances drought stress tolerance in wheat. BMC Plant Biol. 2020; 20(1):259. PMC: 7275420. DOI: 10.1186/s12870-020-02474-5. View

19.

Liang G, Zhang H, Li X, Ai Q, Yu D . bHLH transcription factor bHLH115 regulates iron homeostasis in Arabidopsis thaliana. J Exp Bot. 2017; 68(7):1743-1755. PMC: 5441899. DOI: 10.1093/jxb/erx043. View

20.

Vogel J, Zarka D, Van Buskirk H, Fowler S, Thomashow M . Roles of the CBF2 and ZAT12 transcription factors in configuring the low temperature transcriptome of Arabidopsis. Plant J. 2005; 41(2):195-211. DOI: 10.1111/j.1365-313X.2004.02288.x. View