Serum Expression Level of MicroRNA-122 and Its Significance in Patients with Hepatitis B Virus Infection

Overview

Journal J Healthc Eng

Specialty Biomedical Engineering

Date 2022 Mar 7

PMID 35251580

Authors

Qingqing Fang

Wei Chen

Yourong Jian

Yu Li

Wei Lian

Hongyu Wan

Shiyao Chen

Feng Li

Ying Chen

Affiliations

Soon will be listed here.

Abstract

Objectives: To analyze the expression of miR-122 and evaluate its significance in patients with HBV infection in different phases.

Methods: Eleven chronic hepatitis B (CHB), 26 hepatitis B virus (HBV)-induced cirrhosis, 16 HBV-associated hepatocellular carcinoma (HCC) patients and 10 healthy control cases were enrolled. The serum levels of miR-122 were detected by RT-PCR and compared between healthy individuals and CHB at different stages.

Results: Compared with healthy control cases, serum miR-122 levels were markedly increased in HBV infection cases (AUC = 0.795, =0.002). In the CHB group, miR-122 levels were positively associated with albumin levels ( < 0.05) but had no significant associations with alanine aminotransferase (ALT) and aspartate aminotransferase (AST) ( > 0.05). In the cirrhosis group, miR-122 expression was remarkably lower in the Child C group in comparison with the Child A group (=0.025). At the same time, miR-122 amounts had a negative correlation with HVPG ( < 0.05). In the HCC group, miR-122 amounts were negatively associated with alkaline phosphatase (AKP) and alpha-fetoprotein (AFP) ( < 0.05). Serum miR-122 amounts in 3 patients who died were lower than the survival group (5.520 ± 0.522 vs. 5.860 ± 1.183, > 0.05).

Conclusion: Serum miR-122 can be leveraged to screen patients with HBV infection. In HBV sufferers, the serum miR-122 expression level is related to liver disease progression, hence making it an underlying molecular biomarker for predicting the development of CHB.

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