Use of Extended Protocols with Nonstandard Stimuli to Characterize Rod and Cone Contributions to the Canine Electroretinogram

Overview

Journal Doc Ophthalmol

Specialty Ophthalmology

Date 2022 Mar 5

PMID 35247111

Authors

Nate Pasmanter

Laurence M Occelli

Andras M Komaromy

Simon M Petersen-Jones

Affiliations

Soon will be listed here.

Abstract

Purpose: In this study, we assessed several extended electroretinographic protocols using nonstandard stimuli. Our aim was to separate and quantify the contributions of different populations of retinal cells to the overall response, both to assess normal function and characterize dogs with inherited retinal disease.

Methods: We investigated three different protocols for measuring the full-field flash electroretinogram-(1) chromatic dark-adapted red and blue flashes, (2) increasing luminance blue-background, (3) flicker with fixed frequency and increasing luminance, and flicker with increasing frequency at a fixed luminance-to assess rod and cone contributions to electroretinograms recorded in phenotypically normal control dogs and dogs lacking rod function.

Results: Temporal separation of the rod- and cone-driven responses is possible in the fully dark-adapted eye using dim red flashes. A- and b-wave amplitudes decrease at different rates with increasing background luminance in control dogs. Flicker responses elicited with extended flicker protocols are well fit with mathematical models in control dogs. Dogs lacking rod function demonstrated larger amplitude dark-adapted compared to light-adapted flicker responses.

Conclusions: Using extended protocols of the full-field electroretinogram provides additional characterization of the health and function of different populations of cells in the normal retina and enables quantifiable comparison between phenotypically normal dogs and those with retinal disease.

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References

Kremers J, Jertila M, Link B, Pangeni G, Horn F . Spectral characteristics of the PhNR in the full-field flash electroretinogram of normals and glaucoma patients. Doc Ophthalmol. 2012; 124(2):79-90. DOI: 10.1007/s10633-011-9304-z. View

Tanimoto N, Sothilingam V, Kondo M, Biel M, Humphries P, Seeliger M . Electroretinographic assessment of rod- and cone-mediated bipolar cell pathways using flicker stimuli in mice. Sci Rep. 2015; 5:10731. PMC: 5377071. DOI: 10.1038/srep10731. View

Rufiange M, Rousseau S, Dembinska O, Lachapelle P . Cone-dominated ERG luminance-response function: the Photopic Hill revisited. Doc Ophthalmol. 2002; 104(3):231-48. DOI: 10.1023/a:1015265812018. View

ARMINGTON J . A component of the human electroretinogram associated with red color vision. J Opt Soc Am. 1952; 42(6):393-401. DOI: 10.1364/josa.42.000393. View

Sidjanin D, Lowe J, McElwee J, Milne B, Phippen T, Sargan D . Canine CNGB3 mutations establish cone degeneration as orthologous to the human achromatopsia locus ACHM3. Hum Mol Genet. 2002; 11(16):1823-33. DOI: 10.1093/hmg/11.16.1823. View

Peachey N, Alexander K, Fishman G . Visual adaptation and the cone flicker electroretinogram. Invest Ophthalmol Vis Sci. 1991; 32(5):1517-22. View

Coile D, Pollitz C, Smith J . Behavioral determination of critical flicker fusion in dogs. Physiol Behav. 1989; 45(6):1087-92. DOI: 10.1016/0031-9384(89)90092-9. View

Dunn F, Doan T, Sampath A, Rieke F . Controlling the gain of rod-mediated signals in the Mammalian retina. J Neurosci. 2006; 26(15):3959-70. PMC: 6673884. DOI: 10.1523/JNEUROSCI.5148-05.2006. View

Annear M, Bartoe J, Barker S, Smith A, Curran P, Bainbridge J . Gene therapy in the second eye of RPE65-deficient dogs improves retinal function. Gene Ther. 2010; 18(1):53-61. PMC: 3381842. DOI: 10.1038/gt.2010.111. View

10.

Tuntivanich N, Pittler S, Fischer A, Omar G, Kiupel M, Weber A . Characterization of a canine model of autosomal recessive retinitis pigmentosa due to a PDE6A mutation. Invest Ophthalmol Vis Sci. 2008; 50(2):801-13. PMC: 3720143. DOI: 10.1167/iovs.08-2562. View

11.

Normann R, Werblin F . Control of retinal sensitivity. I. Light and dark adaptation of vertebrate rods and cones. J Gen Physiol. 1974; 63(1):37-61. PMC: 2203542. DOI: 10.1085/jgp.63.1.37. View

12.

Peachey N, Alexander K, Fishman G, Derlacki D . Properties of the human cone system electroretinogram during light adaptation. Appl Opt. 2010; 28(6):1145-50. DOI: 10.1364/AO.28.001145. View

13.

Perlman I, Normann R . Light adaptation and sensitivity controlling mechanisms in vertebrate photoreceptors. Prog Retin Eye Res. 1998; 17(4):523-63. DOI: 10.1016/s1350-9462(98)00005-6. View

14.

Maehara S, Osawa A, Itoh N, Wakaiki S, Tsuzuki K, Seno T . Detection of cone dysfunction induced by digoxin in dogs by multicolor electroretinography. Vet Ophthalmol. 2005; 8(6):407-13. DOI: 10.1111/j.1463-5224.2005.00415.x. View

15.

Hood D, Birch D . Light adaptation of human rod receptors: the leading edge of the human a-wave and models of rod receptor activity. Vision Res. 1993; 33(12):1605-18. DOI: 10.1016/0042-6989(93)90027-t. View

16.

Donner K . Noise and the absolute thresholds of cone and rod vision. Vision Res. 1992; 32(5):853-66. DOI: 10.1016/0042-6989(92)90028-h. View

17.

Aguirre G . Retinal degenerations in the dog. I. Rod dysplasia. Exp Eye Res. 1978; 26(3):233-53. DOI: 10.1016/0014-4835(78)90072-6. View

18.

Brigell M, Jeffrey B, Mahroo O, Tzekov R . ISCEV extended protocol for derivation and analysis of the strong flash rod-isolated ERG a-wave. Doc Ophthalmol. 2020; 140(1):5-12. DOI: 10.1007/s10633-019-09740-4. View

19.

Seeliger M, Brombas A, Weiler R, Humphries P, Knop G, Tanimoto N . Modulation of rod photoreceptor output by HCN1 channels is essential for regular mesopic cone vision. Nat Commun. 2011; 2:532. DOI: 10.1038/ncomms1540. View

20.

Perlman I, Kondo M, Chelva E, Robson A, Holder G . ISCEV extended protocol for the S-cone ERG. Doc Ophthalmol. 2019; 140(2):95-101. DOI: 10.1007/s10633-019-09730-6. View