LiverClear: A Versatile Protocol for Mouse Liver Tissue Clearing

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2022 Mar 4

PMID 35243370

Authors

Laura M Molina

Yekaterina Krutsenko

Nathaniel E C Jenkins

Megan C Smith

Junyan Tao

Travis B Wheeler

Simon C Watkins

Alan M Watson

Satdarshan P Monga

Affiliations

Soon will be listed here.

Abstract

Although there are numerous tissue clearing protocols, most are inadequate for clearing liver tissue. Here we present a flexible protocol for mouse liver tissue; we combine strategies from several previously published protocols for delipidation, decolorization, staining, and refractive index matching. LiverClear is sufficiently versatile to allow clearing of healthy and diseased mouse liver followed by immunofluorescence staining and imaging to visualize intact 3D structures such as bile ducts and hepatocyte canaliculi. We also adapted this protocol for clearing human livers. For complete details on the use and execution of this protocol, please refer to Molina et al. (2021).

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