Grafting Watermelon Onto Pumpkin Increases Chilling Tolerance by Up Regulating to Increase Putrescine Biosynthesis

Overview

Journal Front Plant Sci

Date 2022 Mar 4

PMID 35242149

Authors

Junyang Lu

Fei Cheng

Yuan Huang

Zhilong Bie

Affiliations

Soon will be listed here.

Abstract

Low temperature is a major environmental factor that severely impairs plant growth and productivity. Watermelon () is a chilling-sensitive crop. Grafting of watermelon onto pumpkin rootstock is an effective technique to increase the chilling tolerance of watermelon when exposure to short-time chilling stress. However, the mechanism by which pumpkin rootstock increases chilling tolerance remains poorly understood. Under 10°C/5°C (day/night) chilling stress treatment, pumpkin-grafted watermelon seedlings showed higher chilling tolerance than self-grafted watermelon plants with significantly reduced lipid peroxidation and chilling injury (CI) index. Physiological analysis revealed that pumpkin rootstock grafting led to the notable accumulation of putrescine in watermelon seedlings under chilling conditions. Pre-treat foliar with 1 mM D-arginine (inhibitor of arginine decarboxylase, ADC) increased the electrolyte leakage (EL) of pumpkin-grafted watermelon leaves under chilling stress. This result can be ascribed to the decrease in transcript levels of , , , and genes involved in the synthesis and metabolism of polyamines. Transcriptome analysis showed that pumpkin rootstock improved chilling tolerance in watermelon seedlings by regulating differential gene expression under chilling stress. Pumpkin-grafted seedling reduced the number and expression level of differential genes in watermelon scion under chilling stress. It specifically increased the up-regulated expression of (), a key gene in the polyamine metabolism pathway, and ultimately promoted the accumulation of putrescine. In conclusion, pumpkin rootstock grafting increased the chilling tolerance of watermelon through transcription adjustments, up regulating the expression level of , and promoting the synthesis of putrescine, which ultimately improved the chilling tolerance of pumpkin-grafted watermelon plants.

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References

Li H, Guo Y, Lan Z, Xu K, Chang J, Ahammed G . Methyl jasmonate mediates melatonin-induced cold tolerance of grafted watermelon plants. Hortic Res. 2021; 8(1):57. PMC: 7943586. DOI: 10.1038/s41438-021-00496-0. View

Liu W, Zhang R, Xiang C, Zhang R, Wang Q, Wang T . Transcriptomic and Physiological Analysis Reveal That α-Linolenic Acid Biosynthesis Responds to Early Chilling Tolerance in Pumpkin Rootstock Varieties. Front Plant Sci. 2021; 12:669565. PMC: 8104029. DOI: 10.3389/fpls.2021.669565. View

Shi H, Chan Z . Improvement of plant abiotic stress tolerance through modulation of the polyamine pathway. J Integr Plant Biol. 2014; 56(2):114-21. DOI: 10.1111/jipb.12128. View

Alet A, Sanchez D, Cuevas J, Del Valle S, Altabella T, Tiburcio A . Putrescine accumulation in Arabidopsis thaliana transgenic lines enhances tolerance to dehydration and freezing stress. Plant Signal Behav. 2011; 6(2):278-86. PMC: 3121989. DOI: 10.4161/psb.6.2.14702. View

Thomashow M . PLANT COLD ACCLIMATION: Freezing Tolerance Genes and Regulatory Mechanisms. Annu Rev Plant Physiol Plant Mol Biol. 2004; 50:571-599. DOI: 10.1146/annurev.arplant.50.1.571. View

Chen D, Shao Q, Yin L, Younis A, Zheng B . Polyamine Function in Plants: Metabolism, Regulation on Development, and Roles in Abiotic Stress Responses. Front Plant Sci. 2019; 9:1945. PMC: 6335389. DOI: 10.3389/fpls.2018.01945. View

Landi M . Commentary to: "Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds" by Hodges et al., Planta (1999) 207:604-611. Planta. 2017; 245(6):1067. DOI: 10.1007/s00425-017-2699-3. View

Chen C, Chen H, Zhang Y, Thomas H, Frank M, He Y . TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data. Mol Plant. 2020; 13(8):1194-1202. DOI: 10.1016/j.molp.2020.06.009. View

Liu J, Ban Y, Wen X, Nakajima I, Moriguchi T . Molecular cloning and expression analysis of an arginine decarboxylase gene from peach (Prunus persica). Gene. 2008; 429(1-2):10-7. DOI: 10.1016/j.gene.2008.10.003. View

10.

Gao C, Sheteiwy M, Han J, Dong Z, Pan R, Guan Y . Polyamine biosynthetic pathways and their relation with the cold tolerance of maize ( L.) seedlings. Plant Signal Behav. 2020; 15(11):1807722. PMC: 7588226. DOI: 10.1080/15592324.2020.1807722. View

11.

Kasukabe Y, He L, Nada K, Misawa S, Ihara I, Tachibana S . Overexpression of spermidine synthase enhances tolerance to multiple environmental stresses and up-regulates the expression of various stress-regulated genes in transgenic Arabidopsis thaliana. Plant Cell Physiol. 2004; 45(6):712-22. DOI: 10.1093/pcp/pch083. View

12.

Li M, Wang C, Shi J, Zhang Y, Liu T, Qi H . Abscisic acid and putrescine synergistically regulate the cold tolerance of melon seedlings. Plant Physiol Biochem. 2021; 166:1054-1064. DOI: 10.1016/j.plaphy.2021.07.011. View

13.

Schweikert K, Burritt D . Polyamines in macroalgae: advances and future perspectives. J Phycol. 2016; 51(5):838-49. DOI: 10.1111/jpy.12325. View

14.

Sengupta M, Raychaudhuri S . Partial alleviation of oxidative stress induced by gamma irradiation in Vigna radiata by polyamine treatment. Int J Radiat Biol. 2017; 93(8):803-817. DOI: 10.1080/09553002.2017.1321807. View

15.

Zhang Z, Zheng Y, Ham B, Chen J, Yoshida A, Kochian L . Vascular-mediated signalling involved in early phosphate stress response in plants. Nat Plants. 2016; 2:16033. DOI: 10.1038/nplants.2016.33. View

16.

Wu H, Fu B, Sun P, Xiao C, Liu J . A NAC Transcription Factor Represses Putrescine Biosynthesis and Affects Drought Tolerance. Plant Physiol. 2016; 172(3):1532-1547. PMC: 5100760. DOI: 10.1104/pp.16.01096. View

17.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

18.

Yin X, Yang Y, Lv Y, Li Y, Yang D, Yue Y . BrrICE1.1 is associated with putrescine synthesis through regulation of the arginine decarboxylase gene in freezing tolerance of turnip (Brassica rapa var. rapa). BMC Plant Biol. 2020; 20(1):504. PMC: 7641815. DOI: 10.1186/s12870-020-02697-6. View

19.

Shen W, Nada K, Tachibana S . Involvement of polyamines in the chilling tolerance of cucumber cultivars. Plant Physiol. 2000; 124(1):431-9. PMC: 59156. DOI: 10.1104/pp.124.1.431. View

20.

Song J, Wu H, He F, Qu J, Wang Y, Li C . Citrus sinensis CBF1 Functions in Cold Tolerance by Modulating Putrescine Biosynthesis through Regulation of Arginine Decarboxylase. Plant Cell Physiol. 2021; 63(1):19-29. DOI: 10.1093/pcp/pcab135. View