Oral Intragastric DMBA Administration Induces Acute Lymphocytic Leukemia and Other Tumors in Male Wistar Rats

Overview

Journal J Exp Pharmacol

Publisher Dove Medical Press

Date 2022 Mar 4

PMID 35241938

Authors

Omar S El-Masry

Hussain Abdullah Alhawaj

Muaz O Fagere

Amani Y Owaidah

Aisha Alamri

Khaldoon Alsamman

Affiliations

Soon will be listed here.

Abstract

Background: Animal models of blood cancer are important tools to study these malignancies and also screen for novel therapeutic agents. Evidence from past research on the carcinogenic properties of 7,12-dimethylbenz[a]-anthracene (DMBA) was provided by a handful of studies. However, recent literature on DMBA carcinogenic activity and the underlying mechanisms is scarce.

Objective: The aim of this study was to develop a chemical model of leukemia using DMBA. Male Wistar rats (6 weeks old) were administered 1.5 mg of DMBA dissolved in sesame oil in biweekly doses using oral intragastric intubation.

Materials And Methods: Frequent complete blood counts and blood smear morphology assessment were used to assess the development of leukemia, while gross pathology and histopathology staining were used to evaluate malignancy development.

Results: The results showed that only 4% of rats developed acute lymphocytic leukemia. Interestingly, 36% of the rats developed tumors (parotid tumors [24%] and fibrosarcomas [12%]).

Conclusion: These results suggest the pleiotropic potential of DMBA in the induction of multiple types of malignancies, including leukemia. This could be used as a model to validate therapeutic targets for leukemia and other induced malignancies.

Citing Articles

OBSERVE: guidelines for the refinement of rodent cancer models.

De Vleeschauwer S, van de Ven M, Oudin A, Debusschere K, Connor K, Byrne A Nat Protoc. 2024; 19(9):2571-2596.

PMID: 38992214 DOI: 10.1038/s41596-024-00998-w.

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