Efficacy of Two Endectoparasiticide Products Combining Fipronil and (S)-methoprene or Esafoxolaner with Eprinomectin and Praziquantel Against Fleas and Intestinal Helminths in Cats Naturally Infested in Brazil

Overview

Journal Parasite

Specialty Parasitology

Date 2022 Mar 3

PMID 35238771

Authors

Andre A Cutolo

Debora T G Jardina

Gabriela G De Vito

Cristiano Grisi do Nascimento

Maycon Junior Heidmann

Jose Dirceu Ferreira Nantes

Karolyne Vieira Bassetto

Isabella Cristina Chagas

Edgar Ferreira Pereira Junior

Bruno Gomes de Castro

Eric Tielemans

Affiliations

Soon will be listed here.

Abstract

Eprinomectin and praziquantel, nematodicide and cestodicide compounds, are both combined with the insecticide and acaricide compounds fipronil and (S)-methoprene in Frontline Protect/Broadline, or esafoxolaner in NexGard Combo. These topical feline endectoparasiticide products were tested for efficacy against fleas and intestinal helminths in a field trial in Brazil. Flea- and/or helminth-infested domestic cats were treated twice at a monthly interval following label instructions: 160 cats with Frontline Protect/Broadline and 165 cats with NexGard Combo. The flea and intestinal helminth infestations were evaluated using comb counts and copromicroscopy, respectively before first treatment for baseline value, then 9 and 30 days after each treatment for fleas, and 9 days after each treatment for helminths. Multiparasitism was very frequent at baseline, as amongst the 325 included cats, 295, 280, 86 and 93 cats were at least infested with Ctenocephalides fleas, Ancylostoma, Toxocara and Dipylidium caninum, respectively. Efficacies were calculated by comparing the geometric means at baseline and at post-treatment timepoints for each parasite genus/species. Inclusive of both products and of all evaluation timepoints, the Ctenocephalides, Ancylostoma, Toxocara and D. caninum efficacies were at least 98.3%, 99.8%, 99.8% and 96.3%, respectively. No adverse reactions were observed, except for a few instances of mild, transient, and self-resolving hypersalivation occurring on the day of treatment in both groups. This field trial demonstrated high-level efficacy of Frontline Protect/Broadline and NexGard Combo against major parasites of cats in Brazil.

References

Little S, Adolph C, Downie K, Snider T, Reichard M . High Prevalence of Covert Infection With Gastrointestinal Helminths in Cats. J Am Anim Hosp Assoc. 2015; 51(6):359-64. DOI: 10.5326/JAAHA-MS-6221. View

Mendes-de-Almeida F, Crissiuma A, Gershony L, Willi L, Paiva J, Guerrero J . Characterization of ectoparasites in an urban cat (Felis catus Linnaeus, 1758) population of Rio de Janeiro, Brazil. Parasitol Res. 2010; 108(6):1431-5. DOI: 10.1007/s00436-010-2189-z. View

Marchiondo A, Holdsworth P, Fourie L, Rugg D, Hellmann K, Snyder D . World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) second edition: guidelines for evaluating the efficacy of parasiticides for the treatment, prevention and control of flea and tick infestations on dogs and cats. Vet Parasitol. 2013; 194(1):84-97. DOI: 10.1016/j.vetpar.2013.02.003. View

Traversa D . Pet roundworms and hookworms: a continuing need for global worming. Parasit Vectors. 2012; 5:91. PMC: 3418564. DOI: 10.1186/1756-3305-5-91. View

Penagos-Tabares F, Lange M, Chaparro-Gutierrez J, Taubert A, Hermosilla C . Angiostrongylus vasorum and Aelurostrongylus abstrusus: Neglected and underestimated parasites in South America. Parasit Vectors. 2018; 11(1):208. PMC: 5870519. DOI: 10.1186/s13071-018-2765-0. View

Vercruysse J, Holdsworth P, Letonja T, Conder G, Hamamoto K, Okano K . International harmonisation of anthelmintic efficacy guidelines (Part 2). Vet Parasitol. 2002; 103(4):277-97. DOI: 10.1016/s0304-4017(01)00615-x. View

Knaus M, Baker C, Alva R, Mitchell E, Irwin J, Shukullari E . Efficacy of a novel topical combination of esafoxolaner, eprinomectin and praziquantel in cats against Toxocara cati and Dipylidium caninum. Parasite. 2021; 28:28. PMC: 8019557. DOI: 10.1051/parasite/2021024. View

Rehbein S, Capari B, Duscher G, Keidane D, Kirkova Z, Petkevicius S . Efficacy against nematode and cestode infections and safety of a novel topical fipronil, (S)-methoprene, eprinomectin and praziquantel combination product in domestic cats under field conditions in Europe. Vet Parasitol. 2014; 202(1-2):10-7. DOI: 10.1016/j.vetpar.2014.02.032. View

Morelli S, Diakou A, Colombo M, Di Cesare A, Barlaam A, Dimzas D . Cat Respiratory Nematodes: Current Knowledge, Novel Data and Warranted Studies on Clinical Features, Treatment and Control. Pathogens. 2021; 10(4). PMC: 8069686. DOI: 10.3390/pathogens10040454. View

10.

Lucio-Forster A, Bowman D . Prevalence of fecal-borne parasites detected by centrifugal flotation in feline samples from two shelters in upstate New York. J Feline Med Surg. 2011; 13(4):300-3. PMC: 10832825. DOI: 10.1016/j.jfms.2010.12.013. View

11.

Dantas-Torres F, Otranto D . Dogs, cats, parasites, and humans in Brazil: opening the black box. Parasit Vectors. 2014; 7:22. PMC: 3914713. DOI: 10.1186/1756-3305-7-22. View

12.

Ramos D, Scheremeta R, Oliveira A, Sinkoc A, de Campos Pacheco R . Survey of helminth parasites of cats from the metropolitan area of Cuiabá, Mato Grosso, Brazil. Rev Bras Parasitol Vet. 2013; 22(2):201-6. DOI: 10.1590/S1984-29612013000200040. View

13.

Nagamori Y, Payton M, Duncan-Decocq R, Johnson E . Fecal survey of parasites in free-roaming cats in northcentral Oklahoma, United States. Vet Parasitol Reg Stud Reports. 2019; 14:50-53. DOI: 10.1016/j.vprsr.2018.08.008. View

14.

Calvete C, Lucientes J, Castillo J, Estrada R, Gracia M, Peribanez M . Gastrointestinal helminth parasites in stray cats from the mid-Ebro Valley, Spain. Vet Parasitol. 1998; 75(2-3):235-40. DOI: 10.1016/s0304-4017(97)00182-9. View

15.

Tielemans E, Erasmus H, Momberg M, Pfefferkorn A, Targa N, Chilakapati J . Safety evaluation of a novel topical combination of esafoxolaner, eprinomectin and praziquantel, in reproducing female cats. Parasite. 2021; 28:20. PMC: 8019565. DOI: 10.1051/parasite/2021016. View

16.

Gupta A, Baker C, Wang H, Targa N, Pfefferkorn A, Tielemans E . Target animal safety evaluation of a novel topical combination of esafoxolaner, eprinomectin and praziquantel for cats. Parasite. 2021; 28:18. PMC: 8019570. DOI: 10.1051/parasite/2021015. View

17.

Tielemans E, Otsuki T, Cheesman T, Selmes F, Pfefferkorn A, Prullage J . Efficacy of a novel topical combination of esafoxolaner, eprinomectin and praziquantel against fleas in cats, under field conditions. Parasite. 2021; 28:22. PMC: 8019569. DOI: 10.1051/parasite/2021018. View

18.

Labarthe N, Serrao M, Ferreira A, Almeida N, Guerrero J . A survey of gastrointestinal helminths in cats of the metropolitan region of Rio de Janeiro, Brazil. Vet Parasitol. 2004; 123(1-2):133-9. DOI: 10.1016/j.vetpar.2004.06.002. View

19.

Vercruysse J, Holdsworth P, Letonja T, Barth D, Conder G, Hamamoto K . International harmonisation of anthelmintic efficacy guidelines. Vet Parasitol. 2001; 96(3):171-93. DOI: 10.1016/s0304-4017(00)00443-x. View

20.

Knaus M, Abu-Madi M, Ibarra-Velarde F, Kok D, Kusi I, Postoli R . Efficacy of a novel topical fipronil, (S)-methoprene, eprinomectin and praziquantel combination against naturally acquired intestinal nematode and cestode infections in cats. Vet Parasitol. 2014; 202(1-2):18-25. DOI: 10.1016/j.vetpar.2014.02.033. View