An Organ-derived Extracellular Matrix Triggers in Situ Kidney Regeneration in a Preclinical Model

Overview

Journal NPJ Regen Med

Date 2022 Mar 1

PMID 35228532

Authors

Kazuki Tajima

Hiroshi Yagi

Toshinori Morisaku

Kotaro Nishi

Hiroko Kushige

Hideaki Kojima

Hisanobu Higashi

Kohei Kuroda

Minoru Kitago

Shungo Adachi

Tohru Natsume

Kumiko Nishimura

Mototsugu Oya

Yuko Kitagawa

Affiliations

Soon will be listed here.

Abstract

It has not been considered that nephrons regenerate in adult mammals. We present that an organ-derived extracellular matrix in situ induces nephron regeneration in a preclinical model. A porcine kidney-derived extracellular matrix was sutured onto the surface of partial nephrectomy (PN)-treated kidney. Twenty-eight days after implantation, glomeruli, vessels, and renal tubules, characteristic of nephrons, were histologically observed within the matrix. No fibrillogenesis was observed in the matrix nor the matrix-sutured kidney, although this occurred in a PN kidney without the matrix, indicating the structures were newly induced by the matrix. The expression of renal progenitor markers, including Sall1, Six2, and WT-1, within the matrix supported the induction of nephron regeneration by the matrix. Furthermore, active blood flow was observed inside the matrix using computed tomography. The matrix provides structural and functional foundations for the development of cell-free scaffolds with a remarkably low risk of immune rejection and cancerization.

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