Conjugation of RNA 2'-OH Acylation: Mechanisms Determining Nucleotide Reactivity

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 Feb 28

PMID 35226025

Authors

Biswarup Jash

Eric T Kool

Affiliations

Soon will be listed here.

Abstract

The acylation reactivity of RNA 2'-OH groups has proven broadly useful for labeling and mapping RNA. Here we perform kinetics studies to test the mechanisms governing this reaction, and we find strong steric and inductive effects modulating reactivity. The results shed light on new strategies for improved conjugation and mapping.

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