Matrix Models. Essential Tools for Microscopic Cytochemical Research

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1986 Jan 1

PMID 3522493

Citations 3

Authors

M VAN DER PLOEG

W A Duijndam

Affiliations

Soon will be listed here.

Abstract

An overview is given of the preparation and use of model systems for cytochemistry, dealing with quantitative as well as qualitative aspects. Descriptions are given of the various possibilities to prepare cytochemical matrix models, ranging from macroscopic and microscopic films, to models with more cell-like dimensions as agarose beads, artificial cells and erythrocyte ghosts. Such models allow the study of a large variety of cytochemical processes. Their potentialities are demonstrated in a number of specific applications, comprising: the study of the influence of fixation on cellular processes, reaction specificity and reaction kinetics, quality of reagents and biochemical calibration in cytochemical staining; factors influencing localization of the specific endproduct in enzyme cytochemistry; immunocytochemistry and hybridocytochemistry.

Citing Articles

Antigen retrieval immunohistochemistry: review and future prospects in research and diagnosis over two decades.

Shi S, Shi Y, Taylor C J Histochem Cytochem. 2011; 59(1):13-32.

PMID: 21339172 PMC: 3201121. DOI: 10.1369/jhc.2010.957191.

Standardization of the Feulgen-Schiff technique. Staining characteristics of pure fuchsin dyes; a cytophotometric investigation.

Schulte E, WITTEKIND D Histochemistry. 1989; 91(4):321-31.

PMID: 2732097 DOI: 10.1007/BF00493008.

Quantification of the histochemical reaction for alkaline phosphatase activity using the indoxyl-tetranitro BT method.

Van Noorden C, Jonges G Histochem J. 1987; 19(2):94-102.

PMID: 2438256 DOI: 10.1007/BF01682753.

References

Gahrton G . MICROSPECTROPHOTOMETRIC QUANTITATION OF THE PERIODIC ACID-SCHIFF (PAS) REACTION IN HUMAN NEUTROPHIL LEUKOCYTES BASED ON A MODEL SYSTEM OF GLYCOGEN MICRODROPLETS. Exp Cell Res. 1964; 34:488-506. DOI: 10.1016/0014-4827(64)90234-4. View

VAN DUIJN P, Pascoe E, VAN DER PLOEG M . Theoretical and experimental aspects of enzyme determination in a cytochemical model system of polyacrylamide films containing alkaline phosphatase. J Histochem Cytochem. 1967; 15(11):631-45. DOI: 10.1177/15.11.631. View

HARDONK M, VAN DUIJN . SYNTHESIS AND PROPERTIES OF MODEL SYSTEMS, WITH THEIR USE IN STUDYING THE SCHIFF REACTION IN HISTOCHEMISTRY. J Histochem Cytochem. 1964; 12:533-7. DOI: 10.1177/12.7.533. View

Blakesley R, BOEZI J . A new staining technique for proteins in polyacrylamide gels using coomassie brilliant blue G250. Anal Biochem. 1977; 82(2):580-2. DOI: 10.1016/0003-2697(77)90197-x. View

Persijn J, VAN DUIJN P . Studies of the Feulgen reaction with the aid of DNA incorporated cellulose films. Z Zellforch Microsk Anat Histochem. 1961; 2:283-97. DOI: 10.1007/BF00736505. View

Wenk H, Krug H, Fletcher A . [Microspectrophotometric method for quantitative acetylcholinesterase activity determination in tissue sections]. Acta Histochem. 1973; 45(1):37-60. View

de Jong A, Hak T, VAN DUIJN P, DAEMS W . A new dynamic model system for the study of capture reactions for diffusable compounds in cytochemistry. III. Influence of the matrix composition on the lead phosphate precipitation process in acid phosphatase cytochemistry. Histochem J. 1979; 11(2):163-71. DOI: 10.1007/BF01002993. View

Goldman R, Lenhoff H . Glucose-6-phosphate dehydrogenase adsorbed on collodion membranes. Biochim Biophys Acta. 1971; 242(2):514-8. DOI: 10.1016/0005-2744(71)90246-4. View

Oud P, Henderik J, Huysmans A, Pahlplatz M, Hermkens H, Tas J . The use of Light Green and Orange II as quantitative protein stains, and their combination with the Feulgen method for the simultaneous determination of protein and DNA. Histochemistry. 1984; 80(1):49-57. DOI: 10.1007/BF00492771. View

10.

Tas J, Mendelson D, Noorden C . Cuprolinic Blue: a specific dye for single-stranded RNA in the presence of magnesium chloride. I. Fundamental aspects. Histochem J. 1983; 15(8):801-14. DOI: 10.1007/BF01003343. View

11.

Redi C, VAN DER PLOEG M . Quantitative aspects of the cytochemical Feulgen-DNA procedure studied on model systems and cell nuclei. Histochemistry. 1980; 69(1):1-17. DOI: 10.1007/BF00508362. View

12.

Millar D, Williams E . A step-wedge standard for the quantification of immunoperoxidase techniques. Histochem J. 1982; 14(4):609-20. DOI: 10.1007/BF01011893. View

13.

Barrnett R, Seligman A . Histochemical demonstration of sulfhydryl and disulfide groups of protein. J Natl Cancer Inst. 1954; 14(4):769-803. View

14.

Van Dalen J, VAN DUIJN P . Quantitative aspects of the ninhydrin-Schiff reaction on amino cellulose films and tissue sections. Histochemie. 1971; 26(2):180-9. DOI: 10.1007/BF00293506. View

15.

Hopwood D . Fixatives and fixation: a review. Histochem J. 1969; 1(4):323-60. DOI: 10.1007/BF01003278. View

16.

Cohn N . A model system analysis of the parameters in histone staining. I. Alkaline Fast Green. Histochem J. 1973; 5(6):529-45. DOI: 10.1007/BF01012059. View

17.

Axen R, Porath J . Chemical coupling of enzymes to cross-linked dextran ('Sephadex'). Nature. 1966; 210(5034):367-9. DOI: 10.1038/210367a0. View

18.

Goldman R, Silman H, Caplan S, Kedem O, KATCHALSKI E . Papain membrane on a collodion matrix: preparation and enzymic behavior. Science. 1965; 150(3697):758-60. DOI: 10.1126/science.150.3697.758. View

19.

Wilson R, Kay G, Lilly M . The preparation and kinetics of lactate dehydrogenase attached to water-insoluble particles and sheets. Biochem J. 1968; 108(5):845-53. PMC: 1198891. DOI: 10.1042/bj1080845. View

20.

Kelly J, Carlson L . Protein droplets, especially gelatin, hemoglobin and histone, as microscopic standards for quantitation of cytochemical reactions. Exp Cell Res. 1963; 30:106-24. DOI: 10.1016/0014-4827(63)90218-0. View