MiR-500a-3p is a Potential Prognostic Biomarker in Hepatocellular Carcinoma

Overview

Journal Int J Gen Med

Publisher Dove Medical Press

Specialty General Medicine

Date 2022 Feb 28

PMID 35221718

Authors

Jianting Long

Baoxian Liu

Zhijia Yao

Huiwen Weng

Heping Li

Chunlin Jiang

Shi Fang

Affiliations

Soon will be listed here.

Abstract

Purpose: miR-500a-3p has been extensively reported to be implicated in the development and progression in several human cancer types. This study aimed to investigate the diagnostic and prognostic significance of miR-500a-3p as a biomarker in hepatocellular carcinoma (HCC).

Methods: miR-500a-3p expression was evaluated by in situ hybridization (ISH) and real-time PCR in 10 adjacent normal tissues (ANT), 21 liver fibrosis tissues, and 110 HCC tissues. Statistical analysis was used to investigate the correlation of miR-500a-3p expression with clinicopathological features in HCC patients. Kaplan-Meier survival analysis was performed to evaluate the prognostic significance of miR-500a-3p in overall survival and recurrence-free survival in HCC patients.

Results: In this study, we found that expression levels of miR-500a-3p were enhanced in HCC tissues. High miR-500a-3p levels were positively correlated with multiple clinicopathological features, including advanced clinical stage, distant metastatic status, increased AFP levels and poor tumor differentiation degree. More importantly, high miR-500a-3p levels predicted poor overall survival and early recurrence in HCC patients. Finally, a strong and positive correlation of miR-500a-3p mRNA expression with ISH staining scores was observed in clinical HCC tissues.

Conclusion: Our findings suggest that miR-500a-3p might be used as a novel biomarker to facilitate early diagnosis and predict prognosis in HCC patients.

Citing Articles

Cancer-associated fibroblast exosomes promote prostate cancer metastasis through miR-500a-3p/FBXW7/HSF1 axis under hypoxic microenvironment.

Liu Z, Lin Z, Jiang M, Zhu G, Xiong T, Cao F Cancer Gene Ther. 2024; 31(5):698-709.

PMID: 38351137 DOI: 10.1038/s41417-024-00742-2.

Construction of a Necroptosis-Related miRNA Signature for Predicting the Prognosis of Patients With Hepatocellular Carcinoma.

Meng T, Wang Q, Yang Y, Ren Y, Shi Y Front Genet. 2022; 13:825261.

PMID: 35495130 PMC: 9039163. DOI: 10.3389/fgene.2022.825261.

References

Han Z, Chen H, Fan J, Wu J, Tang H, Peng Z . Up-regulation of microRNA-155 promotes cancer cell invasion and predicts poor survival of hepatocellular carcinoma following liver transplantation. J Cancer Res Clin Oncol. 2011; 138(1):153-61. DOI: 10.1007/s00432-011-1076-z. View

Zhou J, Yu L, Gao X, Hu J, Wang J, Dai Z . Plasma microRNA panel to diagnose hepatitis B virus-related hepatocellular carcinoma. J Clin Oncol. 2011; 29(36):4781-8. DOI: 10.1200/JCO.2011.38.2697. View

Lin H, Zhang L, Zhang C, Liu P . Exosomal MiR-500a-3p promotes cisplatin resistance and stemness via negatively regulating FBXW7 in gastric cancer. J Cell Mol Med. 2020; 24(16):8930-8941. PMC: 7417713. DOI: 10.1111/jcmm.15524. View

Wa Q, Li L, Lin H, Peng X, Ren D, Huang Y . Downregulation of miR‑19a‑3p promotes invasion, migration and bone metastasis via activating TGF‑β signaling in prostate cancer. Oncol Rep. 2017; 39(1):81-90. PMC: 5783607. DOI: 10.3892/or.2017.6096. View

Zhang Z, Ge S, Wang X, Yuan Q, Yan Q, Ye H . Serum miR-483-5p as a potential biomarker to detect hepatocellular carcinoma. Hepatol Int. 2015; 7(1):199-207. DOI: 10.1007/s12072-012-9341-z. View

Ren D, Yang Q, Dai Y, Guo W, Du H, Song L . Oncogenic miR-210-3p promotes prostate cancer cell EMT and bone metastasis via NF-κB signaling pathway. Mol Cancer. 2017; 16(1):117. PMC: 5504657. DOI: 10.1186/s12943-017-0688-6. View

Siegel R, Miller K, Jemal A . Cancer statistics, 2020. CA Cancer J Clin. 2020; 70(1):7-30. DOI: 10.3322/caac.21590. View

Islakoglu Y, Noyan S, Aydos A, Gur Dedeoglu B . Meta-microRNA Biomarker Signatures to Classify Breast Cancer Subtypes. OMICS. 2018; 22(11):709-716. DOI: 10.1089/omi.2018.0157. View

Liao X, Xie Z, Guan C . MiRNA-500a-3p inhibits cell proliferation and invasion by targeting lymphocyte antigen 6 complex locus K (LY6K) in human non-small cell lung cancer. Neoplasma. 2018; 65(5):673-682. DOI: 10.4149/neo_2018_170516N355. View

10.

Jiang C, Long J, Liu B, Xu M, Wang W, Xie X . miR-500a-3p promotes cancer stem cells properties via STAT3 pathway in human hepatocellular carcinoma. J Exp Clin Cancer Res. 2017; 36(1):99. PMC: 5532790. DOI: 10.1186/s13046-017-0568-3. View

11.

Tomimaru Y, Eguchi H, Nagano H, Wada H, Kobayashi S, Marubashi S . Circulating microRNA-21 as a novel biomarker for hepatocellular carcinoma. J Hepatol. 2011; 56(1):167-75. DOI: 10.1016/j.jhep.2011.04.026. View

12.

Qiu Z, Li H, Zhang Z, Zhu Z, He S, Wang X . A Pharmacogenomic Landscape in Human Liver Cancers. Cancer Cell. 2019; 36(2):179-193.e11. PMC: 7505724. DOI: 10.1016/j.ccell.2019.07.001. View

13.

Wong C, Tsang F, Ng I . Non-coding RNAs in hepatocellular carcinoma: molecular functions and pathological implications. Nat Rev Gastroenterol Hepatol. 2018; 15(3):137-151. DOI: 10.1038/nrgastro.2017.169. View

14.

Cosar E, Mamillapalli R, Sahin Ersoy G, Cho S, Seifer B, Taylor H . Serum microRNAs as diagnostic markers of endometriosis: a comprehensive array-based analysis. Fertil Steril. 2016; 106(2):402-9. DOI: 10.1016/j.fertnstert.2016.04.013. View

15.

Yang D, Feng L, Dougherty C, Luker K, Chen D, Cauble M . In vivo targeting of metastatic breast cancer via tumor vasculature-specific nano-graphene oxide. Biomaterials. 2016; 104:361-71. PMC: 5644339. DOI: 10.1016/j.biomaterials.2016.07.029. View

16.

Edens B, Vissers C, Su J, Arumugam S, Xu Z, Shi H . FMRP Modulates Neural Differentiation through mA-Dependent mRNA Nuclear Export. Cell Rep. 2019; 28(4):845-854.e5. PMC: 6687293. DOI: 10.1016/j.celrep.2019.06.072. View

17.

Liu A, Yao T, Wang W, Wong K, Lee N, Fan S . Circulating miR-15b and miR-130b in serum as potential markers for detecting hepatocellular carcinoma: a retrospective cohort study. BMJ Open. 2012; 2(2):e000825. PMC: 3308260. DOI: 10.1136/bmjopen-2012-000825. View

18.

Jiang C, Yu M, Xie X, Huang G, Peng Y, Ren D . miR-217 targeting DKK1 promotes cancer stem cell properties via activation of the Wnt signaling pathway in hepatocellular carcinoma. Oncol Rep. 2017; 38(4):2351-2359. DOI: 10.3892/or.2017.5924. View

19.

Zhang X, Ren D, Wu X, Lin X, Ye L, Lin C . miR-1266 Contributes to Pancreatic Cancer Progression and Chemoresistance by the STAT3 and NF-κB Signaling Pathways. Mol Ther Nucleic Acids. 2018; 11:142-158. PMC: 5842289. DOI: 10.1016/j.omtn.2018.01.004. View

20.

Kim Y, Park S, Lee Y, Kong H, Park J . miRNAs involved in LY6K and estrogen receptor α contribute to tamoxifen-susceptibility in breast cancer. Oncotarget. 2016; 7(27):42261-42273. PMC: 5173133. DOI: 10.18632/oncotarget.9950. View