Shewanella Jiangmenensis Sp. Nov., Isolated from Aquaculture Water

A Gram-stain-negative and facultatively anaerobic bacterial strain designated as JM162201 was isolated from aquaculture water for farming Pacific white shrimp (Litopenaeus vannamei). The genome size of strain JM162201 was 4,436,316 bp, and the genomic DNA G + C content was 55.0%. Phylogenetic analysis based on 16S rRNA gene sequences and genomes showed that strain JM162201 belonged to the genus Shewanella and was closely related to Shewanella litorisediminis SMK1-12 (97.1%), Shewanella khirikhana TH2012 (97.0%), and Shewanella amazonensis SB2B (96.0%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain JM162201 and three reference type strains were below the recognized thresholds of 95.0-96.0% (for ANI) and 70.0% (for dDDH) for species delineation. Growth occurred at 10-40 °C (optimum, 30 °C), at pH 4.0-10.0 (optimum, 7.0-8.0), and in 0-6.0% NaCl (w/v, optimum, 0-0.1%). The major cellular fatty acids of strain JM162201 were summed feature 3 (C ω7c and/or C ω6c), C ω8c, iso-C, C, and C. The predominant quinones were MK7, Q-7, and Q-8. The major polar lipids were phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). Based on the polyphasic taxonomical analyses, strain JM162201 represents a novel species of the genus Shewanella, for which the name Shewanella jiangmenensis sp. nov. is proposed, with the type strain JM162201 (= GDMCC 1.2006 = KCTC 82340).