Virome Analysis Reveals Diverse and Divergent RNA Viruses in Wild Insect Pollinators in Beijing, China

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2022 Feb 26

PMID 35215821

Authors

Nannan Li

Yizhao Huang

Wei Li

Shufa Xu

Affiliations

Soon will be listed here.

Abstract

Insect pollinators provide major pollination services for wild plants and crops. Honeybee viruses can cause serious damage to honeybee colonies. However, viruses of other wild pollinating insects have yet to be fully explored. In the present study, we used RNA sequencing to investigate the viral diversity of 50 species of wild pollinating insects. A total of 3 pathogenic honeybee viruses, 8 previously reported viruses, and 26 novel viruses were identified in sequenced samples. Among these, 7 novel viruses were shown to be closely related to honeybee pathogenic viruses, and 4 were determined to have potential pathogenicity for their hosts. The viruses detected in wild insect pollinators were mainly from the order and the families , , , and . Our study expanded the species range of known insect pollinator viruses, contributing to future efforts to protect economic honeybees and wild pollinating insects.

Citing Articles

Characterisation of the virome of Kieffer (Diptera: Ceratopogonidae) a vector of bluetongue virus in Australia.

Sharpe S, Madhav M, Klein M, Blasdell K, Paradkar P, Lynch S J Gen Virol. 2025; 106(2).

PMID: 39976626 PMC: 11842880. DOI: 10.1099/jgv.0.002076.

The Viromes of Six Ecosystem Service Provider Parasitoid Wasps.

Caldas-Garcia G, Santos V, Fonseca P, de Almeida J, Costa M, Aguiar E Viruses. 2023; 15(12).

PMID: 38140687 PMC: 10747428. DOI: 10.3390/v15122448.

Annual (2023) taxonomic update of RNA-directed RNA polymerase-encoding negative-sense RNA viruses (realm : kingdom : phylum ).

Kuhn J, Abe J, Adkins S, Alkhovsky S, Avsic-Zupanc T, Ayllon M J Gen Virol. 2023; 104(8).

PMID: 37622664 PMC: 10721048. DOI: 10.1099/jgv.0.001864.

Black queen cell virus detected in Canadian mosquitoes.

Baril C, LeMoine C, Cassone B J Insect Sci. 2023; 23(2).

PMID: 37004145 PMC: 10066845. DOI: 10.1093/jisesa/iead016.

Virome Analysis of Normal and Growth Retardation Disease-Affected Macrobrachium rosenbergii.

Zhou D, Liu S, Guo G, He X, Xing C, Miao Q Microbiol Spectr. 2022; 10(6):e0146222.

PMID: 36445118 PMC: 9769563. DOI: 10.1128/spectrum.01462-22.

References

Santamaria J, Villalobos E, Brettell L, Nikaido S, Graham J, Martin S . Evidence of Varroa-mediated deformed wing virus spillover in Hawaii. J Invertebr Pathol. 2017; 151:126-130. DOI: 10.1016/j.jip.2017.11.008. View

McMenamin A, Flenniken M . Recently identified bee viruses and their impact on bee pollinators. Curr Opin Insect Sci. 2018; 26:120-129. DOI: 10.1016/j.cois.2018.02.009. View

Dolezal A, Carrillo-Tripp J, Judd T, Miller W, Bonning B, Toth A . Interacting stressors matter: diet quality and virus infection in honeybee health. R Soc Open Sci. 2019; 6(2):181803. PMC: 6408407. DOI: 10.1098/rsos.181803. View

Marchler-Bauer A, Derbyshire M, Gonzales N, Lu S, Chitsaz F, Geer L . CDD: NCBI's conserved domain database. Nucleic Acids Res. 2014; 43(Database issue):D222-6. PMC: 4383992. DOI: 10.1093/nar/gku1221. View

Charlebois R, Sathiamoorthy S, Logvinoff C, Gisonni-Lex L, Mallet L, Ng S . Sensitivity and breadth of detection of high-throughput sequencing for adventitious virus detection. NPJ Vaccines. 2020; 5(1):61. PMC: 7368052. DOI: 10.1038/s41541-020-0207-4. View

Yoshioka A, Mishima Y, Fukasawa K . Pollinators and Other Flying Insects inside and outside the Fukushima Evacuation Zone. PLoS One. 2015; 10(11):e0140957. PMC: 4641662. DOI: 10.1371/journal.pone.0140957. View

Cornman R . Relative abundance and molecular evolution of Lake Sinai Virus (Sinaivirus) clades. PeerJ. 2019; 7:e6305. PMC: 6431542. DOI: 10.7717/peerj.6305. View

Fijen T, Scheper J, Boom T, Janssen N, Raemakers I, Kleijn D . Insect pollination is at least as important for marketable crop yield as plant quality in a seed crop. Ecol Lett. 2018; 21(11):1704-1713. DOI: 10.1111/ele.13150. View

Giacobino A, Molineri A, Pacini A, Fondevila N, Pietronave H, Rodriguez G . Varroa destructor and viruses association in honey bee colonies under different climatic conditions. Environ Microbiol Rep. 2016; 8(3):407-12. DOI: 10.1111/1758-2229.12410. View

10.

Weaver D, Cantarel B, Elsik C, Boncristiani D, Evans J . Multi-tiered analyses of honey bees that resist or succumb to parasitic mites and viruses. BMC Genomics. 2021; 22(1):720. PMC: 8493683. DOI: 10.1186/s12864-021-08032-z. View

11.

Singh R, Levitt A, Rajotte E, Holmes E, Ostiguy N, vanEngelsdorp D . RNA viruses in hymenopteran pollinators: evidence of inter-Taxa virus transmission via pollen and potential impact on non-Apis hymenopteran species. PLoS One. 2011; 5(12):e14357. PMC: 3008715. DOI: 10.1371/journal.pone.0014357. View

12.

Zhang J, Zhang Y, Han R . The high-throughput production of dsRNA against sacbrood virus for use in the honey bee Apis cerana (Hymenoptera: Apidae). Virus Genes. 2016; 52(5):698-705. DOI: 10.1007/s11262-016-1346-6. View

13.

Corlett R . Flower visitors and pollination in the Oriental (Indomalayan) Region. Biol Rev Camb Philos Soc. 2004; 79(3):497-532. DOI: 10.1017/s1464793103006341. View

14.

Schoonvaere K, Smet L, Smagghe G, Vierstraete A, Braeckman B, de Graaf D . Unbiased RNA Shotgun Metagenomics in Social and Solitary Wild Bees Detects Associations with Eukaryote Parasites and New Viruses. PLoS One. 2016; 11(12):e0168456. PMC: 5179009. DOI: 10.1371/journal.pone.0168456. View

15.

Vasilakis N, Castro-Llanos F, Widen S, Aguilar P, Guzman H, Guevara C . Arboretum and Puerto Almendras viruses: two novel rhabdoviruses isolated from mosquitoes in Peru. J Gen Virol. 2014; 95(Pt 4):787-792. PMC: 3973475. DOI: 10.1099/vir.0.058685-0. View

16.

Caesar L, Cibulski S, Canal C, Blochtein B, Sattler A, Haag K . The virome of an endangered stingless bee suffering from annual mortality in southern Brazil. J Gen Virol. 2019; 100(7):1153-1164. DOI: 10.1099/jgv.0.001273. View

17.

Genersch E, Yue C, Fries I, de Miranda J . Detection of Deformed wing virus, a honey bee viral pathogen, in bumble bees (Bombus terrestris and Bombus pascuorum) with wing deformities. J Invertebr Pathol. 2005; 91(1):61-3. DOI: 10.1016/j.jip.2005.10.002. View

18.

Grabherr M, Haas B, Yassour M, Levin J, Thompson D, Amit I . Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol. 2011; 29(7):644-52. PMC: 3571712. DOI: 10.1038/nbt.1883. View

19.

Traniello I, Bukhari S, Kevill J, Ahmed A, Hamilton A, Naeger N . Meta-analysis of honey bee neurogenomic response links Deformed wing virus type A to precocious behavioral maturation. Sci Rep. 2020; 10(1):3101. PMC: 7033282. DOI: 10.1038/s41598-020-59808-4. View

20.

Gisder S, Genersch E . Viruses of commercialized insect pollinators. J Invertebr Pathol. 2016; 147:51-59. DOI: 10.1016/j.jip.2016.07.010. View