Isolation and Characterization of a Novel Phage and Its Combined Effect with Tigecycline in Controlling Multidrug-Resistant -Associated Skin and Soft Tissue Infections

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2022 Feb 26

PMID 35215788

Authors

Phitchayapak Wintachai

Komwit Surachat

Kamonnut Singkhamanan

Affiliations

Soon will be listed here.

Abstract

Multidrug-resistant (MDR ) is one of the ESKAPE pathogens that restricts available treatment options. MDR is responsible for a dramatic increase in case numbers of a wide variety of infections, including skin and soft tissue infections (SSTIs), resulting in pyoderma, surgical debridement, and necrotizing fasciitis. To investigate an alternative medical treatment for SSTIs, a broad range lytic phage, vB _AbP_ABWU2101 (phage vABWU2101), for lysing MDR in associated SSTIs was isolated and the biological aspects of this phage were investigated. Morphological characterization and genomic analysis revealed that phage vABWU2101 was a new species in the , , family , and order . Antibiofilm activity of phage vABWU2101 demonstrated good activity against both preformed biofilms and biofilm formation. The combination of phage vABWU2101 and tigecycline showed synergistic antimicrobial activities against planktonic and biofilm cells. Scanning electron microscopy confirmed that the antibacterial efficacy of the combination of phage vABWU2101 and tigecycline was more effective than the phage or antibiotic alone. Hence, our findings could potentially be used to develop a therapeutic option for the treatment of SSTIs caused by MDR

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References

Pelyuntha W, Ngasaman R, Yingkajorn M, Chukiatsiri K, Benjakul S, Vongkamjan K . Isolation and Characterization of Potential Phages Targeting Multidrug-Resistant and Major Serovars of Derived From Broiler Production Chain in Thailand. Front Microbiol. 2021; 12:662461. PMC: 8195598. DOI: 10.3389/fmicb.2021.662461. View

Aziz R, Bartels D, Best A, DeJongh M, Disz T, Edwards R . The RAST Server: rapid annotations using subsystems technology. BMC Genomics. 2008; 9:75. PMC: 2265698. DOI: 10.1186/1471-2164-9-75. View

Noskin G . Tigecycline: a new glycylcycline for treatment of serious infections. Clin Infect Dis. 2005; 41 Suppl 5:S303-14. DOI: 10.1086/431672. View

Wintachai P, Naknaen A, Thammaphet J, Pomwised R, Phaonakrop N, Roytrakul S . Characterization of extended-spectrum-β-lactamase producing Klebsiella pneumoniae phage KP1801 and evaluation of therapeutic efficacy in vitro and in vivo. Sci Rep. 2020; 10(1):11803. PMC: 7367294. DOI: 10.1038/s41598-020-68702-y. View

Yan J, Mao J, Mao J, Xie J . Bacteriophage polysaccharide depolymerases and biomedical applications. BioDrugs. 2013; 28(3):265-74. DOI: 10.1007/s40259-013-0081-y. View

Rahman M, Kim S, Kim S, Seol S, Kim J . Characterization of induced Staphylococcus aureus bacteriophage SAP-26 and its anti-biofilm activity with rifampicin. Biofouling. 2011; 27(10):1087-93. DOI: 10.1080/08927014.2011.631169. View

Chan P, Lowe T . tRNAscan-SE: Searching for tRNA Genes in Genomic Sequences. Methods Mol Biol. 2019; 1962:1-14. PMC: 6768409. DOI: 10.1007/978-1-4939-9173-0_1. View

Wintachai P, Naknaen A, Pomwised R, Voravuthikunchai S, Smith D . Isolation and characterization of Siphoviridae phage infecting extensively drug-resistant Acinetobacter baumannii and evaluation of therapeutic efficacy in vitro and in vivo. J Med Microbiol. 2019; 68(7):1096-1108. DOI: 10.1099/jmm.0.001002. View

Pandit A, Adholeya A, Cahill D, Brau L, Kochar M . Microbial biofilms in nature: unlocking their potential for agricultural applications. J Appl Microbiol. 2020; 129(2):199-211. DOI: 10.1111/jam.14609. View

10.

Santamaria R, Bustos P, Sepulveda-Robles O, Lozano L, Rodriguez C, Fernandez J . Narrow-host-range bacteriophages that infect Rhizobium etli associate with distinct genomic types. Appl Environ Microbiol. 2013; 80(2):446-54. PMC: 3911081. DOI: 10.1128/AEM.02256-13. View

11.

Eliopoulos G, Eliopoulos C . Antibiotic combinations: should they be tested?. Clin Microbiol Rev. 1988; 1(2):139-56. PMC: 358039. DOI: 10.1128/CMR.1.2.139. View

12.

Alic S, Naglic T, Tusek-Znidaric M, Ravnikar M, Racki N, Peterka M . Newly Isolated Bacteriophages from the , , and Families Have Variable Effects on Putative Novel spp. Front Microbiol. 2017; 8:1870. PMC: 5626979. DOI: 10.3389/fmicb.2017.01870. View

13.

Mondal S, Akter A, Draper L, Ross R, Hill C . Characterization of an Endolysin Targeting That Affects Spore Outgrowth. Int J Mol Sci. 2021; 22(11). PMC: 8199566. DOI: 10.3390/ijms22115690. View

14.

Gordillo Altamirano F, Barr J . Phage Therapy in the Postantibiotic Era. Clin Microbiol Rev. 2019; 32(2). PMC: 6431132. DOI: 10.1128/CMR.00066-18. View

15.

Oechslin F . Resistance Development to Bacteriophages Occurring during Bacteriophage Therapy. Viruses. 2018; 10(7). PMC: 6070868. DOI: 10.3390/v10070351. View

16.

Perutelli A, Tascini C, Domenici L, Garibaldi S, Baroni C, CECCHI E . Safety and efficacy of tigecycline in complicated and uncomplicated pelvic inflammatory disease. Eur Rev Med Pharmacol Sci. 2018; 22(11):3595-3601. DOI: 10.26355/eurrev_201806_15186. View

17.

Yele A, Thawal N, Sahu P, Chopade B . Novel lytic bacteriophage AB7-IBB1 of Acinetobacter baumannii: isolation, characterization and its effect on biofilm. Arch Virol. 2012; 157(8):1441-50. DOI: 10.1007/s00705-012-1320-0. View

18.

Zurawski D, Banerjee J, Alamneh Y, Shearer J, Demons S . Skin and Soft Tissue Models for Acinetobacter baumannii Infection. Methods Mol Biol. 2019; 1946:271-287. DOI: 10.1007/978-1-4939-9118-1_25. View

19.

Turner D, Ackermann H, Kropinski A, Lavigne R, Sutton J, Reynolds D . Comparative Analysis of 37 Bacteriophages. Viruses. 2018; 10(1). PMC: 5795418. DOI: 10.3390/v10010005. View

20.

Clark W . Comparison of several methods for preserving bacteriophages. Appl Microbiol. 1962; 10:466-71. PMC: 1057894. DOI: 10.1128/am.10.5.466-471.1962. View