Dwarf Pomegranate ( L. Var. ): Source of 5-HMF and Bioactive Compounds with Applications in the Protection of Woody Crops

Overview

Journal Plants (Basel)

Date 2022 Feb 26

PMID 35214882

Authors

Eva Sanchez-Hernandez

Laura Buzon-Duran

Jose A Cuchi-Oterino

Jesus Martin-Gil

Belen Lorenzo-Vidal

Pablo Martin-Ramos

Affiliations

Soon will be listed here.

Abstract

While the properties of edible pomegranate varieties have been widely explored, there is little information on ornamental types. In this study, possible alternatives for the valorization of dwarf pomegranate fruits have been explored. The characterization of their hydromethanolic extract by gas chromatography-mass spectrometry evidenced the presence of high contents of 5-hydroxymethylfurfural (a carbon-neutral feedstock for the production of fuels and other chemicals) and - and -sitosterol stereoisomers. The microbicidal activity of the crude extract, both alone and in a conjugate complex with chitosan oligomers (COS), was investigated against three plant pathogenic microorganisms that cause significant losses in woody crops: , and . In in vitro assays, a strong synergistic behavior was found after conjugation of the bioactive constituents of the fruit extract with COS, resulting in minimum inhibitory concentration (MIC) values of 750 and 375 μg·mL against and , respectively, and an EC value of 993 μg·mL against . Hence, extracts from the non-edible fruits of this may hold promise as a source of high value-added phytochemicals or as environmentally friendly agrochemicals.

Citing Articles

Screening for Novel Beneficial Environmental Bacteria for an Antagonism-Based Biological Control.

Esteban-Herrero G, Alvarez B, Santander R, Biosca E Microorganisms. 2023; 11(7).

PMID: 37512967 PMC: 10383364. DOI: 10.3390/microorganisms11071795.

Phytochemical Profiling of L. Flower and Leaf Extracts and Their Antimicrobial Potential against Almond Tree Pathogens.

Sanchez-Hernandez E, Balduque-Gil J, Gonzalez-Garcia V, Barriuso-Vargas J, Casanova-Gascon J, Martin-Gil J Int J Mol Sci. 2023; 24(2).

PMID: 36674670 PMC: 9866908. DOI: 10.3390/ijms24021154.

Valorization of L. Bark as a Source of Phytochemicals with Antimicrobial Activity against Apple Tree Diseases.

Sanchez-Hernandez E, Gonzalez-Garcia V, Casanova-Gascon J, Barriuso-Vargas J, Balduque-Gil J, Lorenzo-Vidal B Plants (Basel). 2022; 11(24).

PMID: 36559527 PMC: 9785260. DOI: 10.3390/plants11243415.

Multifunctional Nanocarriers Based on Chitosan Oligomers and Graphitic Carbon Nitride Assembly.

Santiago-Aliste A, Sanchez-Hernandez E, Langa-Lomba N, Gonzalez-Garcia V, Casanova-Gascon J, Martin-Gil J Materials (Basel). 2022; 15(24).

PMID: 36556785 PMC: 9785438. DOI: 10.3390/ma15248981.

Natural Antioxidant, Antibacterial, and Antiproliferative Activities of Ethanolic Extracts from L. Tree Barks Mediated by Extracellular Signal-Regulated Kinase.

Leesombun A, Sariya L, Taowan J, Nakthong C, Thongjuy O, Boonmasawai S Plants (Basel). 2022; 11(17).

PMID: 36079640 PMC: 9460874. DOI: 10.3390/plants11172258.

References

Sundarraj S, Thangam R, Sreevani V, Kaveri K, Gunasekaran P, Achiraman S . γ-Sitosterol from Acacia nilotica L. induces G2/M cell cycle arrest and apoptosis through c-Myc suppression in MCF-7 and A549 cells. J Ethnopharmacol. 2012; 141(3):803-9. DOI: 10.1016/j.jep.2012.03.014. View

Amri Z, Zaouay F, Lazreg-Aref H, Soltana H, Mneri A, Mars M . Phytochemical content, Fatty acids composition and antioxidant potential of different pomegranate parts: Comparison between edible and non edible varieties grown in Tunisia. Int J Biol Macromol. 2017; 104(Pt A):274-280. DOI: 10.1016/j.ijbiomac.2017.06.022. View

Kharchoufi S, Parafati L, Licciardello F, Muratore G, Hamdi M, Cirvilleri G . Edible coatings incorporating pomegranate peel extract and biocontrol yeast to reduce Penicillium digitatum postharvest decay of oranges. Food Microbiol. 2018; 74:107-112. DOI: 10.1016/j.fm.2018.03.011. View

Buzon-Duran L, Martin-Gil J, Perez-Lebena E, Ruano-Rosa D, Revuelta J, Casanova-Gascon J . Antifungal Agents Based on Chitosan Oligomers, ε-polylysine and spp. Secondary Metabolites against Three Botryosphaeriaceae Species. Antibiotics (Basel). 2019; 8(3). PMC: 6783921. DOI: 10.3390/antibiotics8030099. View

Ay C, Ozcan A, ErdoGan Y, Ozcan A . Characterization of Punica granatum L. peels and quantitatively determination of its biosorption behavior towards lead(II) ions and Acid Blue 40. Colloids Surf B Biointerfaces. 2012; 100:197-204. DOI: 10.1016/j.colsurfb.2012.05.013. View

Wafa B, Makni M, Ammar S, Khannous L, Hassana A, Bouaziz M . Antimicrobial effect of the Tunisian Nana variety Punica granatum L. extracts against Salmonella enterica (serovars Kentucky and Enteritidis) isolated from chicken meat and phenolic composition of its peel extract. Int J Food Microbiol. 2016; 241:123-131. DOI: 10.1016/j.ijfoodmicro.2016.10.007. View

Curro S, Caruso M, Distefano G, Gentile A, La Malfa S . New microsatellite loci for pomegranate, Punica granatum (Lythraceae). Am J Bot. 2011; 97(7):e58-60. DOI: 10.3732/ajb.1000143. View

El Deeb K, Eid H, Ali Z, Shams M, Elfiky A . Bioassay-guided fractionation and identification of antidiabetic compounds from the rind of Var. nana. Nat Prod Res. 2019; 35(12):2103-2106. DOI: 10.1080/14786419.2019.1655411. View

da Silva Lacerda V, Lopez-Sotelo J, Correa-Guimaraes A, Hernandez-Navarro S, Sanchez-Bascones M, Navas-Gracia L . A kinetic study on microwave-assisted conversion of cellulose and lignocellulosic waste into hydroxymethylfurfural/furfural. Bioresour Technol. 2015; 180:88-96. DOI: 10.1016/j.biortech.2014.12.089. View

10.

Tayel A, Moussa S, Salem M, Mazrou K, El-Tras W . Control of citrus molds using bioactive coatings incorporated with fungal chitosan/plant extracts composite. J Sci Food Agric. 2015; 96(4):1306-12. DOI: 10.1002/jsfa.7223. View

11.

Pangallo S, Li Destri Nicosia M, Agosteo G, Abdelfattah A, Romeo F, Cacciola S . Evaluation of a Pomegranate Peel Extract as an Alternative Means to Control Olive Anthracnose. Phytopathology. 2017; 107(12):1462-1467. DOI: 10.1094/PHYTO-04-17-0133-R. View

12.

Fischer U, Carle R, Kammerer D . Thermal stability of anthocyanins and colourless phenolics in pomegranate (Punica granatum L.) juices and model solutions. Food Chem. 2013; 138(2-3):1800-9. DOI: 10.1016/j.foodchem.2012.10.072. View

13.

Arendrup M, Cuenca-Estrella M, Lass-Florl C, Hope W . EUCAST technical note on the EUCAST definitive document EDef 7.2: method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for yeasts EDef 7.2 (EUCAST-AFST). Clin Microbiol Infect. 2012; 18(7):E246-7. DOI: 10.1111/j.1469-0691.2012.03880.x. View

14.

Heber D, Seeram N, Wyatt H, Henning S, Zhang Y, Ogden L . Safety and antioxidant activity of a pomegranate ellagitannin-enriched polyphenol dietary supplement in overweight individuals with increased waist size. J Agric Food Chem. 2007; 55(24):10050-4. DOI: 10.1021/jf071689v. View

15.

Rosas-Burgos E, Burgos-Hernandez A, Noguera-Artiaga L, Kacaniova M, Hernandez-Garcia F, Cardenas-Lopez J . Antimicrobial activity of pomegranate peel extracts as affected by cultivar. J Sci Food Agric. 2016; 97(3):802-810. DOI: 10.1002/jsfa.7799. View

16.

Pirzadeh M, Caporaso N, Rauf A, Shariati M, Yessimbekov Z, Khan M . Pomegranate as a source of bioactive constituents: a review on their characterization, properties and applications. Crit Rev Food Sci Nutr. 2020; 61(6):982-999. DOI: 10.1080/10408398.2020.1749825. View

17.

Garcia R, Pizarro C, Lavin A, Bueno J . Characterization of Spanish biomass wastes for energy use. Bioresour Technol. 2011; 103(1):249-58. DOI: 10.1016/j.biortech.2011.10.004. View