PCLAF Promotes Neuroblastoma G1/S Cell Cycle Progression Via the E2F1/PTTG1 Axis

Overview

Journal Cell Death Dis

Specialties Cell Biology
General Medicine

Date 2022 Feb 25

PMID 35210406

Authors

Xiaowei Liu

Yuanxia Cai

Cheng Cheng

Yaoyao Gu

Xiaoxiao Hu

Kai Chen

Yeming Wu

Zhixiang Wu

Affiliations

Soon will be listed here.

Abstract

PCLAF (PCNA clamp-associated factor), also known as PAF15/ KIAA0101, is overexpressed in most human cancers and is a predominant regulator of tumor progression. However, its biological function in neuroblastoma remains unclear. PCLAF is extremely overexpressed in neuroblastoma and is associated with poor prognosis. Through the analysis of various data sets, we found that the high expression of PCLAF is positively correlated with increased stage and high risk of neuroblastoma. Most importantly, knocking down PCLAF could restrict the proliferation of neuroblastoma cells in vitro and in vitro. By analyzing RNA-seq data, we found that the enrichment of cell cycle-related pathway genes was most significant among the differentially expressed downregulated genes after reducing the expression of PCLAF. In addition, PCLAF accelerated the G1/S transition of the neuroblastoma cell cycle by activating the E2F1/PTTG1 signaling pathway. In this study, we reveal the mechanism by which PCLAF facilitates cell cycle progression and recommend that the PCLAF/E2F1/PTTG1 axis is a therapeutic target in neuroblastoma.

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