Morpho-Molecular Characterization of Five Novel Taxa in Parabambusicolaceae (Massarineae, Pleosporales) from Yunnan, China

Parabambusicolaceae is a well-studied family in Massarineae, Pleosporales, comprising nine genera and approximately 16 species. The family was introduced to accommodate saprobic bambusicola-like species in both freshwater and terrestrial environments that mostly occur on bamboos and grasses but are also found on different host substrates. In the present study, we surveyed and collected ascomycetes from bamboo and submerged grass across Yunnan Province, China. A biphasic approach based on morphological characteristics and multigene phylogeny demonstrated five new taxa in Parabambusicolaceae. A novel genus is introduced as a monotypic genus to accommodate sp. nov., collected from dead culms of grass submerged in a freshwater stream. The genus is unique in forming filiform ascospores, which differ from other known genera in Parabambusicolaceae. Multigene phylogeny showed that the genus has a close relationship with . Moreover, the novel monotypic genus , isolated from bamboo, was introduced to accommodate sp. nov. formed a separated branch basal to and with high support (100% ML, 1.00 PP). Furthermore, the newly introduced species, sp. nov. was also isolated from bamboo in terrestrial habitats. clustered with the other three described species and has a close relationship with with significant support (88% ML, 1.00 PP). was reported as the fourth species in this genus. Detailed description, illustration, and updated phylogeny of Parabambusicolaceae were provided.