Phenylpropanoid Glycoside and Phenolic Acid Profiles and Biological Activities of Biomass Extracts from Different Types of Microshoot Cultures and Soil-Grown Plant

Overview

Journal Antioxidants (Basel)

Date 2022 Feb 25

PMID 35204291

Authors

Pawel Kubica

Adam Kokotkiewicz

Magdalena Anna Malinowska

Alicja Synowiec

Malgorzata Gniewosz

Shah Hussain

Muhammad Yaqoob

Gunther K Bonn

Thomas Jakschitz

Eman A Mahmoud

Tarek K Zin El-Abedin

Hosam O Elansary

Maria Luczkiewicz

Halina Ekiert

Agnieszka Szopa

Affiliations

Soon will be listed here.

Abstract

Different types of microshoot cultures (agar, stationary liquid, agitated, and bioreactors) of were optimized for biomass growth and the production of phenylpropanoid glycosides and phenolic acids. Using ultra-high performance liquid chromatography with high-resolution time-of-flight mass spectrometry, the presence of verbascoside, isoverbascoside, leucoseptoside A/isomers, and cistanoside D/isomer was confirmed in the methanolic extracts obtained from all types of in vitro cultures. The compound's content was determined by ultra-high-performance liquid chromatography. The main metabolites in biomass extracts were verbascoside and isoverbascoside (maximum 4881.61 and 451.80 mg/100 g dry weight (DW)). In the soil-grown plant extract, verbascoside was also dominated (1728.97 mg/100 g DW). The content of phenolic acids in the analyzed extracts was below 24 mg/100 g DW. The highest radical scavenging activity was found in the biomass extract from agitated cultures, the most effective reducing power in agar culture extract, and the highest chelating activity in extract from bioreactor cultures. The extracts showed significantly stronger bacteriostatic and bactericidal activity against Gram-positive bacteria (minimum inhibitory concentration (MIC) of 0.3-2.2 mg/mL and minimum bactericidal concentration (MBC) of 0.6-9 mg/mL) than against Gram-negative bacteria (MIC 0.6-9 mg/mL, MBC of 0.6-18 mg/mL). The biomass extract from liquid stationary culture showed the strongest antibacterial activity, while the extract from soil-grown herb had the lowest.

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