Endometrial Cytokine Expression from Clinically Suspected Genital Tuberculosis Patients at Tertiary Care Hospitals in Dhaka

Overview

Journal J Clin Tuberc Other Mycobact Dis

Date 2022 Feb 24

PMID 35198736

Authors

Sheikh Joly Ferdous Ara

Sharmeen Ahmed

Ahmed Abu Saleh

Md Maruf Ahmed Molla

Sharmin Chowdhury

Shaheda Anwar

Affiliations

Soon will be listed here.

Abstract

Objective: The objective of this study was to measure gamma interferon (IFN-γ) and tumor necrosis factor alpha (TNF-α) expression in endometrial tissue and/or aspirate from suspected genital tuberculosis patients with ectopic pregnancy and infertility in Bangladesh.

Methodology: A total 78 women of clinically suspected genital tuberculosis patients were enrolled as study population. These patients underwent manual vaccum aspiration (MVA) procedure, and endometrial tissues and/or aspirates were collected. Ziehl -Neelsen staining (Z-N staining) and Lowen-Stein Jensen (L-J) culture were done to detect . The study participants were categorized as genital tuberculosis positive cases, genital tuberculosis negative cases and presumptive for tuberculosis cases based on the case definition used in this study. TNF-α and IFN-γ were measured by ELISA. Statistical analysis was done using SPSS (version-22).

Results: Out of 78 participants, pro-inflammatory cytokines IFN-γ and TNF-α were significantly increased in TB positive patients than TB negative patients (p < 0.05). IFN-γ value of TB positive patients (41.26 ± 41.05) was higher than TB negative (22.94 ± 44.51) patients. TNF-α value (44.31 ± 64.22) of TB positive patients was higher than TB negative (15.86 ± 41.45) patients. IFN-γ and TNF-α value of presumptive for tuberculosis cases were not statistically significant. According to ROC analysis, cut off value for IFN-γ was 23.5 and for TNF-α was 10 with highest sensitivity and specificity of 66.7%, 89.3%, and 66.7% and 73.1% respectively.

Conclusion: IFN-γ and TNF-α were significantly higher in TB positive patients and it may act as a potential biomarker for diagnosis of genital tuberculosis.

Citing Articles

Diagnosis of Genital Tuberculosis in Infertile Women by Using the Composite Reference Standard.

Saxena R, Shrinet K, Rai S, Singh K, Jain S, Jain S Dis Markers. 2022; 2022:8078639.

PMID: 36016849 PMC: 9398877. DOI: 10.1155/2022/8078639.

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