Gradients of PI(4,5)P and PI(3,5)P Jointly Participate in Shaping the Back State of Cells

Overview

Journal Front Cell Dev Biol

Specialty Cell Biology

Date 2022 Feb 21

PMID 35186938

Authors

Dong Li

FeiFei Sun

Yihong Yang

Hui Tu

Huaqing Cai

Affiliations

Soon will be listed here.

Abstract

Polarity, which refers to the molecular or structural asymmetry in cells, is essential for diverse cellular functions. has proven to be a valuable system for dissecting the molecular mechanisms of cell polarity. Previous studies in have revealed a range of signaling and cytoskeletal proteins that function at the leading edge to promote pseudopod extension and migration. In contrast, how proteins are localized to the trailing edge is not well understood. By screening for asymmetrically localized proteins, we identified a novel trailing-edge protein we named Teep1. We show that a charged surface formed by two pleckstrin homology (PH) domains in Teep1 is necessary and sufficient for targeting it to the rear of cells. Combining biochemical and imaging analyses, we demonstrate that Teep1 interacts preferentially with PI(4,5)P and PI(3,5)P and simultaneous elimination of these lipid species in cells blocks the membrane association of Teep1. Furthermore, a leading-edge localized myotubularin phosphatase likely mediates the removal of PI(3,5)P from the front, as well as the formation of a back-to-front gradient of PI(3,5)P. Together our data indicate that PI(4,5)P and PI(3,5)P on the plasma membrane jointly participate in shaping the back state of cells.

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