Preparation, Characterization, and Evaluation of Liposomes Containing Oridonin from

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Feb 15

PMID 35164121

Authors

Yinyue Wang

Mai Wang

Feier Lin

Xinyan Zhang

Yongming Zhao

Chunyan Guo

Jin Wang

Affiliations

Soon will be listed here.

Abstract

Due to the remarkable anti-tumor activities of oridonin (Ori), research on has attracted more and more attention in the pharmaceutical field. The purpose of this study was to extract Ori from by ultrasound-assisted extraction (UAE) and prepare Ori liposomes as a novel delivery system to improve the bioavailability and biocompatibility. Response surface methodology (RSM), namely Box-Behnken design (BBD), was applied to optimize extraction conditions, formulation, and preparation process. The results demonstrated that the optimal extraction conditions were an ethanol concentration of 75.9%, an extraction time of 35.7 min, and a solid/liquid ratio of 1:32.6. Under these optimal conditions, the extraction yield of Ori was 4.23 mg/g, which was well matched with the predicted value (4.28 mg/g). The optimal preparation conditions of Ori liposomes by RSM, with an ultrasonic time of 41.1 min, a soybean phospholipids/drug ratio of 9.6 g/g, and a water bath temperature of 53.4 °C, had higher encapsulation efficiency (84.1%). The characterization studies indicated that Ori liposomes had well-dispersible spherical shapes and uniform sizes with a particle size of 137.7 nm, a polydispersity index (PDI) of 0.216, and zeta potential of -24.0 mV. In addition, Ori liposomes presented better activity than free Ori. Therefore, the results indicated that Ori liposomes could enhance the bioactivity of Ori, being proposed as a promising vehicle for drug delivery.

Citing Articles

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References

Ren G, Liu D, Guo W, Wang M, Wu C, Guo M . Docetaxel prodrug liposomes for tumor therapy: characterization, in vitro and in vivo evaluation. Drug Deliv. 2016; 23(4):1272-81. DOI: 10.3109/10717544.2016.1165312. View

Mei Y, Xu J, Zhao J, Feng N, Liu Y, Wei L . An HPLC method for determination of oridonin in rabbits using isopsoralen as an internal standard and its application to pharmacokinetic studies for oridonin-loaded nanoparticles. J Chromatogr B Analyt Technol Biomed Life Sci. 2008; 869(1-2):138-41. DOI: 10.1016/j.jchromb.2008.05.005. View

Gonzalez Gomez A, Hosseinidoust Z . Liposomes for Antibiotic Encapsulation and Delivery. ACS Infect Dis. 2020; 6(5):896-908. DOI: 10.1021/acsinfecdis.9b00357. View

Danaei M, Dehghankhold M, Ataei S, Hasanzadeh Davarani F, Javanmard R, Dokhani A . Impact of Particle Size and Polydispersity Index on the Clinical Applications of Lipidic Nanocarrier Systems. Pharmaceutics. 2018; 10(2). PMC: 6027495. DOI: 10.3390/pharmaceutics10020057. View

Wang Q, Wang Y, Huang M, Hayat K, Kurtz N, Wu X . Ultrasound-assisted alkaline proteinase extraction enhances the yield of pecan protein and modifies its functional properties. Ultrason Sonochem. 2021; 80:105789. PMC: 8551211. DOI: 10.1016/j.ultsonch.2021.105789. View

Yang Q, Gan R, Ge Y, Zhang D, Corke A . Ultrasonic Treatment Increases Extraction Rate of Common Bean ( L.) Antioxidants. Antioxidants (Basel). 2019; 8(4). PMC: 6523112. DOI: 10.3390/antiox8040083. View

Lu J, Chen X, Qu S, Yao B, Xu Y, Wu J . Oridonin induces G/M cell cycle arrest and apoptosis via the PI3K/Akt signaling pathway in hormone-independent prostate cancer cells. Oncol Lett. 2017; 13(4):2838-2846. PMC: 5403405. DOI: 10.3892/ol.2017.5751. View

Zhou G, Kang H, Wang L, Gao L, Liu P, Xie J . Oridonin, a diterpenoid extracted from medicinal herbs, targets AML1-ETO fusion protein and shows potent antitumor activity with low adverse effects on t(8;21) leukemia in vitro and in vivo. Blood. 2007; 109(8):3441-50. PMC: 1852250. DOI: 10.1182/blood-2006-06-032250. View

Xia S, Zhang X, Li C, Guan H . Oridonin inhibits breast cancer growth and metastasis through blocking the Notch signaling. Saudi Pharm J. 2017; 25(4):638-643. PMC: 5447451. DOI: 10.1016/j.jsps.2017.04.037. View

10.

Ravar F, Saadat E, Gholami M, Dehghankelishadi P, Mahdavi M, Azami S . Hyaluronic acid-coated liposomes for targeted delivery of paclitaxel, in-vitro characterization and in-vivo evaluation. J Control Release. 2016; 229:10-22. DOI: 10.1016/j.jconrel.2016.03.012. View

11.

Adla S, Tonduru A, Kronenberger T, Kudova E, Poso A, Huttunen K . Neurosteroids: Structure-Uptake Relationships and Computational Modeling of Organic Anion Transporting Polypeptides (OATP)1A2. Molecules. 2021; 26(18). PMC: 8472597. DOI: 10.3390/molecules26185662. View

12.

Wu J, Ding Y, Chen C, Zhou Z, Ding C, Chen H . A new oridonin analog suppresses triple-negative breast cancer cells and tumor growth via the induction of death receptor 5. Cancer Lett. 2016; 380(2):393-402. DOI: 10.1016/j.canlet.2016.06.024. View

13.

Xu L, Bi Y, Xu Y, Zhang Z, Xu W, Zhang S . Oridonin inhibits the migration and epithelial-to-mesenchymal transition of small cell lung cancer cells by suppressing FAK-ERK1/2 signalling pathway. J Cell Mol Med. 2020; 24(8):4480-4493. PMC: 7176879. DOI: 10.1111/jcmm.15106. View

14.

Bu H, Shen F, Cui J . The inhibitory effect of oridonin on colon cancer was mediated by deactivation of TGF-β1/Smads-PAI-1 signaling pathway in vitro and vivo. Onco Targets Ther. 2019; 12:7467-7476. PMC: 6752205. DOI: 10.2147/OTT.S220401. View

15.

He F, Bai Y, Wang J, Wei J, Yu C, Li S . Isolation and purification of oridonin from the whole plant of Isodon rubescens by high-speed counter-current chromatography. Molecules. 2011; 16(9):7949-57. PMC: 6264197. DOI: 10.3390/molecules16097949. View

16.

Li D, Han T, Liao J, Hu X, Xu S, Tian K . Oridonin, a Promising ent-Kaurane Diterpenoid Lead Compound. Int J Mol Sci. 2016; 17(9). PMC: 5037675. DOI: 10.3390/ijms17091395. View

17.

Pretor S, Bartels J, Lorenz T, Dahl K, Finke J, Peterat G . Cellular uptake of coumarin-6 under microfluidic conditions into HCE-T cells from nanoscale formulations. Mol Pharm. 2014; 12(1):34-45. DOI: 10.1021/mp500401t. View

18.

Han B, Yang Y, Chen J, Tang H, Sun Y, Zhang Z . Preparation, Characterization, and Pharmacokinetic Study of a Novel Long-Acting Targeted Paclitaxel Liposome with Antitumor Activity. Int J Nanomedicine. 2020; 15:553-571. PMC: 6986409. DOI: 10.2147/IJN.S228715. View

19.

Luo D, Yi Y, Peng K, Liu T, Yang J, Liu S . Oridonin derivatives as potential anticancer drug candidates triggering apoptosis through mitochondrial pathway in the liver cancer cells. Eur J Med Chem. 2019; 178:365-379. DOI: 10.1016/j.ejmech.2019.06.006. View

20.

Parak K . Rational drug loading of liposomes revisited. J Control Release. 2017; 252:125. DOI: 10.1016/j.jconrel.2017.03.394. View