Phytochemical Characterization, Antioxidant Activity, and Cytotoxicity of Methanolic Leaf Extract of (Green Type) (Thunb.) Jacq

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Feb 15

PMID 35164026

Authors

Igor V Rzhepakovsky

David A Areshidze

Svetlana S Avanesyan

Wolf D Grimm

Natalya V Filatova

Aleksander V Kalinin

Stanislav G Kochergin

Maria A Kozlova

Vladimir P Kurchenko

Marina N Sizonenko

Alexei A Terentiev

Lyudmila D Timchenko

Maria M Trigub

Andrey A Nagdalian

Sergei I Piskov

Affiliations

Soon will be listed here.

Abstract

genus has been extensively studied due to its diverse biological activities. We evaluated the methanolic extract of leaves of (Green type) (Thunb.) Jacques, the species that is less studied compared to . The aim was to identify phytoconstituents of the methanolic extract of leaves of and biological properties of its different fractions. Water fraction was analyzed with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Nineteen compounds belonging to different chemical classes were identified in the methanolic extract of leaves of (Green type) (Thunb.) Jacques. In addition to several fatty acids, isoprenoid and steroid compounds were found among the most abundant constituents. One of the identified compounds, 4'-methylphenyl-1C-sulfonyl-β-d-galactoside, was not detected earlier in extracts. The water fraction was toxic to HeLa cells but not to Vero cells. Our data demonstrate that methanolic extract of leaves of can be a valuable source of bioactive constituents. The water fraction of the extract exhibited promising antitumor potential based on a high ratio of HeLa vs. Vero cytotoxicity.

Citing Articles

Phytochemical analysis, identification of bioactive compounds using GC-MS, and hypoglycemic potential, antioxidant potential, and ADME analysis of root and leaf.

Kavya P, Theijeswini R, Gayathri M Front Chem. 2024; 12:1458505.

PMID: 39345858 PMC: 11427758. DOI: 10.3389/fchem.2024.1458505.

GC-ToF-MS Profiling and In Vitro Inhibitory Effects of Selected South African Plants against Important Mycotoxigenic Phytopathogens.

Mariri N, Dikhoba P, Mongalo N, Makhafola T Life (Basel). 2023; 13(8).

PMID: 37629517 PMC: 10455341. DOI: 10.3390/life13081660.

Exopolymer-Functionalized Nanoselenium from SR41: Characterization, Monosaccharide Analysis and Free Radical Scavenging Ability.

Wang F, Du M, Kai L, Du S, Hu W, Wang Y Polymers (Basel). 2022; 14(17).

PMID: 36080599 PMC: 9459814. DOI: 10.3390/polym14173523.

Anti-Depressant Properties of Crocin Molecules in Saffron.

Siddiqui S, Ali Redha A, Snoeck E, Singh S, Simal-Gandara J, Ibrahim S Molecules. 2022; 27(7).

PMID: 35408474 PMC: 9000812. DOI: 10.3390/molecules27072076.

References

Kangsamaksin T, Chaithongyot S, Wootthichairangsan C, Hanchaina R, Tangshewinsirikul C, Svasti J . Lupeol and stigmasterol suppress tumor angiogenesis and inhibit cholangiocarcinoma growth in mice via downregulation of tumor necrosis factor-α. PLoS One. 2017; 12(12):e0189628. PMC: 5726636. DOI: 10.1371/journal.pone.0189628. View

Severin I, Dumont C, Jondeau-Cabaton A, Graillot V, Chagnon M . Genotoxic activities of the food contaminant 5-hydroxymethylfurfural using different in vitro bioassays. Toxicol Lett. 2009; 192(2):189-94. DOI: 10.1016/j.toxlet.2009.10.022. View

Ray S, Chatterjee K, De D, Ghosh D . Bioefficacy of hydromethanolic extract of tuber of Chlorophytum borivilianum (Safed Musli) for the management of male infertility in cyproterone acetate-treated albino rats. Andrologia. 2013; 46(6):659-71. DOI: 10.1111/and.12133. View

Batta A, Xu G, Honda A, Miyazaki T, Salen G . Stigmasterol reduces plasma cholesterol levels and inhibits hepatic synthesis and intestinal absorption in the rat. Metabolism. 2006; 55(3):292-9. DOI: 10.1016/j.metabol.2005.08.024. View

Liang Q, Yang J, He J, Chen X, Zhang H, Jia M . Stigmasterol alleviates cerebral ischemia/reperfusion injury by attenuating inflammation and improving antioxidant defenses in rats. Biosci Rep. 2020; 40(4). PMC: 7160377. DOI: 10.1042/BSR20192133. View

van Rooijen M, Mensink R . Palmitic Acid Versus Stearic Acid: Effects of Interesterification and Intakes on Cardiometabolic Risk Markers - A Systematic Review. Nutrients. 2020; 12(3). PMC: 7146500. DOI: 10.3390/nu12030615. View

Heikal A, Abd El-Sadek M, Salama A, Taha H . Comparative study between and -derived extracts of cactus ( L. Mill) against prostate and mammary cancer cell lines. Heliyon. 2021; 7(9):e08016. PMC: 8481975. DOI: 10.1016/j.heliyon.2021.e08016. View

Nawaz A, Li E, Khalifa I, Walayat N, Liu J, Nilofar . Effect of Structurally Different Pectin on Dough Rheology, Structure, Pasting and Water Distribution Properties of Partially Meat-Based Sugar Snap Cookies. Foods. 2021; 10(11). PMC: 8625401. DOI: 10.3390/foods10112692. View

Davidson A, Glasscock C, McClanahan D, Benson J, Higgins A . Toxicity Minimized Cryoprotectant Addition and Removal Procedures for Adherent Endothelial Cells. PLoS One. 2015; 10(11):e0142828. PMC: 4659675. DOI: 10.1371/journal.pone.0142828. View

10.

Kumar M, Meena P, Verma S, Kumar M, Kumar A . Anti-tumour, anti-mutagenic and chemomodulatory potential of Chlorophytum borivilianum. Asian Pac J Cancer Prev. 2010; 11(2):327-34. View

11.

Petropoulos S, Fernandes A, Barros L, Ferreira I . A comparison of the phenolic profile and antioxidant activity of different Cichorium spinosum L. ecotypes. J Sci Food Agric. 2017; 98(1):183-189. DOI: 10.1002/jsfa.8453. View

12.

Smith K, Hayat F, Andrews J, Migaud M, Gassman N . Dihydroxyacetone Exposure Alters NAD(P)H and Induces Mitochondrial Stress and Autophagy in HEK293T Cells. Chem Res Toxicol. 2019; 32(8):1722-1731. PMC: 6701868. DOI: 10.1021/acs.chemrestox.9b00230. View

13.

Wang J, Huang M, Yang J, Ma X, Zheng S, Deng S . Anti-diabetic activity of stigmasterol from soybean oil by targeting the GLUT4 glucose transporter. Food Nutr Res. 2017; 61(1):1364117. PMC: 5614214. DOI: 10.1080/16546628.2017.1364117. View

14.

Sharma G, Kumar M . Antioxidant and modulatory role of Chlorophytum borivilianum against arsenic induced testicular impairment. J Environ Sci (China). 2013; 24(12):2159-65. DOI: 10.1016/s1001-0742(11)61019-x. View

15.

Li K, Yuan D, Yan R, Meng L, Zhang Y, Zhu K . Stigmasterol exhibits potent antitumor effects in human gastric cancer cells mediated via inhibition of cell migration, cell cycle arrest, mitochondrial mediated apoptosis and inhibition of JAK/STAT signalling pathway. J BUON. 2018; 23(5):1420-1425. View

16.

Emran T, Rahman M, Uddin M, Rahman M, Uddin M, Dash R . Effects of organic extracts and their different fractions of five Bangladeshi plants on in vitro thrombolysis. BMC Complement Altern Med. 2015; 15:128. PMC: 4414290. DOI: 10.1186/s12906-015-0643-2. View

17.

Nemes D, Kovacs R, Nagy F, Mezo M, Poczok N, Ujhelyi Z . Interaction between Different Pharmaceutical Excipients in Liquid Dosage Forms-Assessment of Cytotoxicity and Antimicrobial Activity. Molecules. 2018; 23(7). PMC: 6100184. DOI: 10.3390/molecules23071827. View

18.

Tao C, Shkumatov A, Alexander S, Ason B, Zhou M . Stigmasterol accumulation causes cardiac injury and promotes mortality. Commun Biol. 2019; 2:20. PMC: 6335236. DOI: 10.1038/s42003-018-0245-x. View

19.

Haque M, Moon I . Stigmasterol upregulates immediate early genes and promotes neuronal cytoarchitecture in primary hippocampal neurons as revealed by transcriptome analysis. Phytomedicine. 2018; 46:164-175. DOI: 10.1016/j.phymed.2018.04.012. View

20.

Juillerat-Jeanneret L, Robert M, Juillerat M . Peptides from Lactobacillus hydrolysates of bovine milk caseins inhibit prolyl-peptidases of human colon cells. J Agric Food Chem. 2010; 59(1):370-7. DOI: 10.1021/jf102803a. View